Detailed information of TA system TA028381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 839039..839724
• Replicon: plasmid pSYMA
• Accession: NC_018683
• Organism: Sinorhizobium meliloti Rm41

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BN406_RS22800
• Protein ID: WP_041169477.1
• Coordinates: 839039..839458 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XET8
• Locus tag: BN406_RS22805
• Protein ID: WP_013845417.1
• Coordinates: 839455..839724 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN406_RS22775	834514..835515	-	1002	WP_014990040.1	IS30 family transposase	-
BN406_RS22780	835799..837156	-	1358	Protein_802	ISNCY family transposase	-
BN406_RS22785	837488..837868	-	381	WP_003526626.1	transcriptional regulator	-
BN406_RS22790	837886..838197	-	312	WP_014528619.1	type II toxin-antitoxin system HigB family toxin	-
BN406_RS22795	838472..838885	+	414	WP_014528620.1	hypothetical protein	-
BN406_RS22800	839039..839458	-	420	WP_041169477.1	type II toxin-antitoxin system VapC family toxin	Toxin
BN406_RS22805	839455..839724	-	270	WP_013845417.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BN406_RS22810	839862..840332	+	471	Protein_808	LysR family transcriptional regulator	-
BN406_RS22815	840683..841429	+	747	Protein_809	oxidoreductase	-
BN406_RS37025	841718..842636	+	919	Protein_810	alpha/beta hydrolase	-
BN406_RS22825	842798..843016	-	219	WP_013845422.1	4-oxalocrotonate tautomerase family protein	-
BN406_RS22830	843038..843778	-	741	WP_013845423.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IIc	htpB	1..1559666	1559666

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14831.14 Da
• Isoelectric Point: 5.1858

>T28381 WP_041169477.1 NC_018683:c839458-839039 [Sinorhizobium meliloti Rm41]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILRSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSG

Nucleotide

• Length: 420 bp

>T28381 NC_018683:c839458-839039 [Sinorhizobium meliloti Rm41]
GTGAGCAGGCTCTACATGCTGGACACCAATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CATCGAAACTACTGGCGCAGATCGAGACCATTCTTCGAAGTATGCAAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCCGCTGGTAAGCCCATCGGCCCGAATGACCTGTTCATCGCCGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACGGCCAACAGTAGCGAGTTCACACGCGTTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTCCTTCGGGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10203.67 Da
• Isoelectric Point: 4.4798

>AT28381 WP_013845417.1 NC_018683:c839724-839455 [Sinorhizobium meliloti Rm41]
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT28381 NC_018683:c839724-839455 [Sinorhizobium meliloti Rm41]
ATGCCCGAACTCCGACACAACGAACCAACACCGCCAAGGGAAGCTAAGCTCTTCCGAAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCCGGCGATCGGGTGATGATCCATCGGGACGGAGATCGGCTGATAATAGAGCCGGTGC
TCCGCAAGAACTTGCTGGAGGTGCTGGCTGGTTTGGAGCCGCTCGGGTCTGAAGATGAGTTCCCCGACGTTGACGAAACC
CTGCTGCCGGTGAAGGAAATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A844A3Y5