Detailed information of TA system TA028378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 67947..68482
• Replicon: plasmid pSYMA
• Accession: NC_018683
• Organism: Sinorhizobium meliloti Rm41

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BN406_RS19125
• Protein ID: WP_014989489.1
• Coordinates: 67947..68222 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2N8JT21
• Locus tag: BN406_RS19130
• Protein ID: WP_013845222.1
• Coordinates: 68222..68482 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN406_RS19100	63335..64282	+	948	WP_014989488.1	LysR family transcriptional regulator	-
BN406_RS35025	64438..64743	-	306	Protein_54	hypothetical protein	-
BN406_RS19110	65456..65893	+	438	WP_013845218.1	VOC family protein	-
BN406_RS19115	66396..66695	+	300	WP_013845219.1	hypothetical protein	-
BN406_RS19120	66744..67919	-	1176	WP_013845220.1	site-specific integrase	-
BN406_RS19125	67947..68222	-	276	WP_014989489.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BN406_RS19130	68222..68482	-	261	WP_013845222.1	ribbon-helix-helix domain-containing protein	Antitoxin
BN406_RS19135	68637..68846	-	210	Protein_60	transposase	-
BN406_RS19140	68854..69273	-	420	WP_013845223.1	type II toxin-antitoxin system VapC family toxin	-
BN406_RS19145	69270..69512	-	243	WP_013845224.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
BN406_RS19150	69735..70883	+	1149	WP_041169439.1	DUF1612 and helix-turn-helix domain-containing protein	-
BN406_RS19155	71073..73040	+	1968	WP_014989492.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IIc	htpB	1..1559666	1559666
	-	flank	IS/Tn	-	-	68637..68864	227

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10895.46 Da
• Isoelectric Point: 9.9707

>T28378 WP_014989489.1 NC_018683:c68222-67947 [Sinorhizobium meliloti Rm41]
MKLEWTSKAVADLGRLYDFLAPVNRQAAARTVQSWTSAPARLLEQPRIGERLEEFDPREVRRILVGHYEIRYEIRQSTIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T28378 NC_018683:c68222-67947 [Sinorhizobium meliloti Rm41]
ATGAAGCTTGAGTGGACGAGTAAGGCAGTCGCCGATCTTGGGCGCCTCTATGATTTCCTGGCACCGGTAAATCGGCAAGC
TGCGGCTCGTACTGTGCAATCATGGACGAGCGCTCCAGCACGCCTGCTCGAACAACCGCGCATCGGCGAACGGCTGGAGG
AATTCGACCCTCGAGAGGTTCGGCGAATTCTCGTTGGCCATTACGAAATACGATATGAGATCCGGCAGTCGACGATCTAC
GTGCTGCGGCTTTGGCATACGCGTGAAGACCGTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9652.01 Da
• Isoelectric Point: 4.8182

>AT28378 WP_013845222.1 NC_018683:c68482-68222 [Sinorhizobium meliloti Rm41]
METKVLTAHVPLPLAEKVDQIAARLERSRGWIVKQALTAWVDQEEERRRLTLEALADVDAGRVIDHQAVQAWADSLDSDK
PLSLPL

Nucleotide

• Length: 261 bp

>AT28378 NC_018683:c68482-68222 [Sinorhizobium meliloti Rm41]
ATGGAAACGAAGGTGCTGACGGCGCACGTACCACTGCCGCTCGCCGAGAAAGTCGATCAGATCGCGGCCCGGCTTGAGCG
CTCGCGCGGGTGGATCGTCAAGCAGGCGCTGACCGCCTGGGTCGATCAGGAAGAGGAGCGCCGACGCTTGACGCTGGAAG
CGCTTGCCGACGTCGACGCTGGCCGCGTTATCGATCACCAGGCCGTCCAGGCCTGGGCCGACAGTCTTGATAGTGACAAG
CCGCTCTCCTTGCCGCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2N8JT21