Detailed information of TA system TA028375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 6891452..6892149
• Replicon: chromosome
• Accession: NC_018681
• Organism: Nocardia brasiliensis ATCC 700358

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: O3I_RS30470
• Protein ID: WP_014986870.1
• Coordinates: 6891452..6891877 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: K0F938
• Locus tag: O3I_RS30475
• Protein ID: WP_014986871.1
• Coordinates: 6891874..6892149 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
O3I_RS30440	6886589..6887878	-	1290	WP_014986864.1	ATP-binding protein	-
O3I_RS30445	6887875..6888558	-	684	WP_041564580.1	response regulator transcription factor	-
O3I_RS30450	6888733..6889146	-	414	WP_051066792.1	flavodoxin family protein	-
O3I_RS30455	6889215..6889718	-	504	WP_014986867.1	hypothetical protein	-
O3I_RS30460	6889750..6890496	-	747	WP_014986868.1	4-hydroxy-tetrahydrodipicolinate reductase	-
O3I_RS43100	6890630..6891451	+	822	WP_014986869.1	alpha/beta hydrolase	-
O3I_RS30470	6891452..6891877	-	426	WP_014986870.1	type II toxin-antitoxin system VapC family toxin	Toxin
O3I_RS30475	6891874..6892149	-	276	WP_014986871.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
O3I_RS45715	6892184..6892621	-	438	WP_014986872.1	hypothetical protein	-
O3I_RS30485	6892704..6893132	+	429	WP_014986873.1	MarR family winged helix-turn-helix transcriptional regulator	-
O3I_RS30490	6893186..6894298	-	1113	WP_014986874.1	alcohol dehydrogenase catalytic domain-containing protein	-
O3I_RS30495	6894350..6895654	-	1305	WP_014986875.1	DUF418 domain-containing protein	-
O3I_RS30500	6895858..6896505	+	648	WP_014986876.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (12-76)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15707.04 Da
• Isoelectric Point: 6.8641

>T28375 WP_014986870.1 NC_018681:c6891877-6891452 [Nocardia brasiliensis ATCC 700358]
VKNYVLDTCAVSEWMKPQPDPGLIRWLHTADEDRLHLSVITLGEIRKGIEKLPDSPKRQRLVEWLTESLLTRFGRRILPV
DAVVAQAWGRLAARLEQGGTSVESADVLIAATAESHGFTVVTRNVKHFEPTGVSLVCPWGE

Nucleotide

• Length: 426 bp

>T28375 NC_018681:c6891877-6891452 [Nocardia brasiliensis ATCC 700358]
GTGAAAAACTACGTTCTCGACACCTGTGCCGTGTCGGAATGGATGAAGCCGCAACCGGATCCGGGCCTCATCCGTTGGTT
GCACACGGCGGATGAGGACCGGCTCCACCTCAGCGTCATCACCCTGGGGGAGATTCGGAAGGGCATCGAGAAGCTACCGG
ATTCGCCCAAGCGGCAGCGGCTGGTGGAGTGGTTGACGGAGTCGTTGCTCACCCGCTTCGGGCGGCGCATCTTGCCGGTC
GATGCGGTTGTGGCCCAAGCCTGGGGACGCCTGGCGGCCAGGCTCGAGCAAGGTGGGACGTCCGTTGAATCGGCGGATGT
GTTGATCGCGGCAACTGCGGAGTCGCACGGCTTCACGGTTGTCACTCGTAATGTGAAGCATTTCGAGCCGACCGGCGTTT
CGCTGGTCTGCCCATGGGGTGAATAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10434.87 Da
• Isoelectric Point: 5.8992

>AT28375 WP_014986871.1 NC_018681:c6892149-6891874 [Nocardia brasiliensis ATCC 700358]
MTRKRPQRPDIVWPLADAKARLSELIDTVEREGPQVISKHGREVAVVVPIDEWDQKTSRKGSLAEFFADSPLRNSGLEIE
RIRSELRDIDL

Nucleotide

• Length: 276 bp

>AT28375 NC_018681:c6892149-6891874 [Nocardia brasiliensis ATCC 700358]
ATGACTCGGAAGCGGCCGCAGCGCCCGGACATCGTGTGGCCGCTCGCCGACGCGAAGGCTCGACTGTCCGAGTTGATCGA
CACCGTAGAGCGGGAGGGGCCTCAAGTGATCAGCAAGCACGGACGTGAAGTCGCGGTAGTCGTGCCCATCGACGAATGGG
ACCAGAAGACAAGCCGGAAGGGCAGTCTCGCGGAGTTCTTCGCCGATTCGCCGCTGCGTAATTCCGGTCTAGAGATCGAA
CGTATCCGGTCGGAACTGCGCGACATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: K0F938