Detailed information of TA system TA283680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1268..1742
• Replicon: plasmid p3_130128
• Accession: NZ_CP127239
• Organism: Klebsiella pneumoniae strain 130128

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: QRA17_RS29180
• Protein ID: WP_004197762.1
• Coordinates: 1268..1534 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: QRA17_RS29185
• Protein ID: WP_000879771.1
• Coordinates: 1518..1742 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QRA17_RS29170 (QRA17_29170)	237..782	+	546	WP_023317533.1	hypothetical protein	-
QRA17_RS29175 (QRA17_29175)	848..1198	+	351	WP_004197756.1	hypothetical protein	-
QRA17_RS29180 (QRA17_29180)	1268..1534	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QRA17_RS29185 (QRA17_29185)	1518..1742	-	225	WP_000879771.1	DUF6290 family protein	Antitoxin
QRA17_RS29190 (QRA17_29190)	1905..2033	+	129	WP_000523570.1	hypothetical protein	-
QRA17_RS29195 (QRA17_29195)	2409..2621	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
QRA17_RS29200 (QRA17_29200)	2628..3137	-	510	WP_032433972.1	MbeB family mobilization protein	-
QRA17_RS29205 (QRA17_29205)	3172..3606	-	435	Protein_8	relaxase/mobilization nuclease domain-containing protein	-
QRA17_RS29210 (QRA17_29210)	3815..4159	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
QRA17_RS29215 (QRA17_29215)	4149..4325	-	177	WP_004197757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5595	5595

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T283680 WP_004197762.1 NZ_CP127239:c1534-1268 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T283680 NZ_CP127239:c1534-1268 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT283680 WP_000879771.1 NZ_CP127239:c1742-1518 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT283680 NZ_CP127239:c1742-1518 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5