Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 35483..36042 | Replicon | plasmid pAA-EA11 |
| Accession | NC_018666 | ||
| Organism | Escherichia coli O104:H4 str. 2011C-3493 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | G4VUF8 |
| Locus tag | O3K_RS26350 | Protein ID | WP_000421273.1 |
| Coordinates | 35483..35758 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0SRR3 |
| Locus tag | O3K_RS26355 | Protein ID | WP_001387467.1 |
| Coordinates | 35758..36042 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| O3K_RS26310 | 30525..30755 | + | 231 | WP_001261288.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | - |
| O3K_RS26315 | 30779..31180 | + | 402 | WP_001392130.1 | type II toxin-antitoxin system VapC family toxin | - |
| O3K_RS26320 | 31263..31412 | - | 150 | Protein_45 | transposase domain-containing protein | - |
| O3K_RS26325 | 32504..33481 | + | 978 | WP_001387465.1 | RepB family plasmid replication initiator protein | - |
| O3K_RS26330 | 33760..34500 | - | 741 | WP_001066951.1 | tyrosine-type recombinase/integrase | - |
| O3K_RS26335 | 34621..34746 | - | 126 | WP_032159539.1 | hypothetical protein | - |
| O3K_RS26340 | 34869..35030 | - | 162 | WP_001440647.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| O3K_RS26345 | 35146..35477 | - | 332 | Protein_50 | type II toxin-antitoxin system ParD family antitoxin | - |
| O3K_RS26350 | 35483..35758 | - | 276 | WP_000421273.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| O3K_RS26355 | 35758..36042 | - | 285 | WP_001387467.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| O3K_RS26360 | 36950..37778 | - | 829 | Protein_53 | incFII family plasmid replication initiator RepA | - |
| O3K_RS26365 | 37771..37845 | - | 75 | WP_014966221.1 | RepA leader peptide Tap | - |
| O3K_RS26800 | 37842..37976 | - | 135 | Protein_55 | protein CopA/IncA | - |
| O3K_RS26370 | 38083..38337 | - | 255 | WP_000083850.1 | replication regulatory protein RepA | - |
| O3K_RS26375 | 38618..39598 | + | 981 | WP_000019440.1 | IS5-like element ISKpn26 family transposase | - |
| O3K_RS26380 | 40049..40261 | - | 213 | Protein_58 | ANR family transcriptional regulator | - |
| O3K_RS26385 | 40326..41003 | + | 678 | WP_001339397.1 | IS66 family insertion sequence hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | aap/aspU / aggA / aggB / aggC / aggD | 1..74217 | 74217 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10765.45 Da Isoelectric Point: 9.9324
>T28365 WP_000421273.1 NC_018666:c35758-35483 [Escherichia coli O104:H4 str. 2011C-3493]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T28365 NC_018666:c35758-35483 [Escherichia coli O104:H4 str. 2011C-3493]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10460.87 Da Isoelectric Point: 5.6558
>AT28365 WP_001387467.1 NC_018666:c36042-35758 [Escherichia coli O104:H4 str. 2011C-3493]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
Download Length: 285 bp
>AT28365 NC_018666:c36042-35758 [Escherichia coli O104:H4 str. 2011C-3493]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y6H1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y880 |