Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 30525..31180 Replicon plasmid pAA-EA11
Accession NC_018666
Organism Escherichia coli O104:H4 str. 2011C-3493

Toxin (Protein)


Gene name vagD Uniprot ID A0A2H3M3N3
Locus tag O3K_RS26315 Protein ID WP_001392130.1
Coordinates 30779..31180 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A0E0YAG4
Locus tag O3K_RS26310 Protein ID WP_001261288.1
Coordinates 30525..30755 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3K_RS26275 25577..25993 - 417 WP_001278815.1 plasmid partitioning/stability family protein -
O3K_RS26280 25986..26966 - 981 WP_000688504.1 plasmid segregation protein ParM -
O3K_RS26285 27380..27688 - 309 WP_000030204.1 molecular chaperone GroEL -
O3K_RS26290 27775..28419 - 645 WP_001144032.1 ParA family protein -
O3K_RS26295 28598..29404 - 807 WP_000016961.1 site-specific integrase -
O3K_RS26300 29405..29710 - 306 WP_001159872.1 type II toxin-antitoxin system toxin CcdB -
O3K_RS26305 29712..29930 - 219 WP_000813638.1 type II toxin-antitoxin system antitoxin CcdA -
O3K_RS26310 30525..30755 + 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
O3K_RS26315 30779..31180 + 402 WP_001392130.1 type II toxin-antitoxin system VapC family toxin Toxin
O3K_RS26320 31263..31412 - 150 Protein_45 transposase domain-containing protein -
O3K_RS26325 32504..33481 + 978 WP_001387465.1 RepB family plasmid replication initiator protein -
O3K_RS26330 33760..34500 - 741 WP_001066951.1 tyrosine-type recombinase/integrase -
O3K_RS26335 34621..34746 - 126 WP_032159539.1 hypothetical protein -
O3K_RS26340 34869..35030 - 162 WP_001440647.1 type II toxin-antitoxin system RelE/ParE family toxin -
O3K_RS26345 35146..35477 - 332 Protein_50 type II toxin-antitoxin system ParD family antitoxin -
O3K_RS26350 35483..35758 - 276 WP_000421273.1 type II toxin-antitoxin system RelE/ParE family toxin -
O3K_RS26355 35758..36042 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aap/aspU / aggA / aggB / aggC / aggD 1..74217 74217


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14647.00 Da        Isoelectric Point: 6.7123

>T28364 WP_001392130.1 NC_018666:30779-31180 [Escherichia coli O104:H4 str. 2011C-3493]
MLDTNICSFIMREQPAAVIKRLEQAVLRNHRIVVSAITYAEMRFDATGPKASPRHIALVDAFCARLDAILPWDRAAVDAT
TEIKMALRLAETPIGPNNTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 402 bp

>T28364 NC_018666:30779-31180 [Escherichia coli O104:H4 str. 2011C-3493]
ATGCTCGACACGAATATCTGCTCTTTCATTATGCGTGAACAGCCCGCCGCAGTGATCAAACGTCTGGAGCAGGCGGTGCT
GCGTAACCACCGCATTGTCGTGTCGGCCATCACCTATGCGGAGATGCGTTTCGACGCCACCGGCCCGAAAGCCTCACCGC
GTCATATTGCGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATTCTGCCCTGGGACCGCGCTGCCGTGGACGCCACC
ACGGAGATTAAAATGGCACTGCGGCTGGCCGAGACGCCAATCGGCCCGAACAACACGGCGATTGCCGGGCACGCCATCGC
TGCCGGGGCGATACTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCTGACCTGGTGCTGGAAGACTGGGTGAAGT
AA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8684.76 Da        Isoelectric Point: 4.3654

>AT28364 WP_001261288.1 NC_018666:30525-30755 [Escherichia coli O104:H4 str. 2011C-3493]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWSSFAALEKADADFMAEREDIVSDEGRFDL

Download         Length: 231 bp

>AT28364 NC_018666:30525-30755 [Escherichia coli O104:H4 str. 2011C-3493]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTTGAGATCGTCCGCGAAGGAGACAGCATTATATTGCGCCCGGTCCGGCCGACCTGGAGCTCGTTTGCGGCACTCG
AAAAGGCCGACGCAGACTTTATGGCGGAGCGTGAGGACATTGTCAGCGACGAAGGAAGATTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H3M3N3

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0YAG4

References