Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 29405..29930 Replicon plasmid pAA-EA11
Accession NC_018666
Organism Escherichia coli O104:H4 str. 2011C-3493

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0E0Y871
Locus tag O3K_RS26300 Protein ID WP_001159872.1
Coordinates 29405..29710 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0E0Y6P9
Locus tag O3K_RS26305 Protein ID WP_000813638.1
Coordinates 29712..29930 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3K_RS26275 25577..25993 - 417 WP_001278815.1 plasmid partitioning/stability family protein -
O3K_RS26280 25986..26966 - 981 WP_000688504.1 plasmid segregation protein ParM -
O3K_RS26285 27380..27688 - 309 WP_000030204.1 molecular chaperone GroEL -
O3K_RS26290 27775..28419 - 645 WP_001144032.1 ParA family protein -
O3K_RS26295 28598..29404 - 807 WP_000016961.1 site-specific integrase -
O3K_RS26300 29405..29710 - 306 WP_001159872.1 type II toxin-antitoxin system toxin CcdB Toxin
O3K_RS26305 29712..29930 - 219 WP_000813638.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
O3K_RS26310 30525..30755 + 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
O3K_RS26315 30779..31180 + 402 WP_001392130.1 type II toxin-antitoxin system VapC family toxin -
O3K_RS26320 31263..31412 - 150 Protein_45 transposase domain-containing protein -
O3K_RS26325 32504..33481 + 978 WP_001387465.1 RepB family plasmid replication initiator protein -
O3K_RS26330 33760..34500 - 741 WP_001066951.1 tyrosine-type recombinase/integrase -
O3K_RS26335 34621..34746 - 126 WP_032159539.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aap/aspU / aggA / aggB / aggC / aggD 1..74217 74217


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11722.51 Da        Isoelectric Point: 5.6919

>T28363 WP_001159872.1 NC_018666:c29710-29405 [Escherichia coli O104:H4 str. 2011C-3493]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHWENDIKNAINLMFWGI

Download         Length: 306 bp

>T28363 NC_018666:c29710-29405 [Escherichia coli O104:H4 str. 2011C-3493]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTAATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACTGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8344.29 Da        Isoelectric Point: 4.5203

>AT28363 WP_000813638.1 NC_018666:c29930-29712 [Escherichia coli O104:H4 str. 2011C-3493]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVAKFIEMNGSFADENRDW

Download         Length: 219 bp

>AT28363 NC_018666:c29930-29712 [Escherichia coli O104:H4 str. 2011C-3493]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAAGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y871

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6P9

References