283621

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 86150..87287 Replicon plasmid p90_2023_A
Accession NZ_CP127178
Organism Enterococcus faecalis strain 90_2023

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag QQS48_RS13550 Protein ID WP_002332783.1
Coordinates 86150..87013 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag QQS48_RS13555 Protein ID WP_002326825.1
Coordinates 87015..87287 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQS48_RS13505 (QQS48_13505) 81392..81502 - 111 Protein_95 aminoglycoside 6-adenylyltransferase -
QQS48_RS13510 (QQS48_13510) 81521..81637 - 117 Protein_96 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
QQS48_RS13515 (QQS48_13515) 81807..82544 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
QQS48_RS13520 (QQS48_13520) 82669..82752 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
QQS48_RS13525 (QQS48_13525) 82801..83040 - 240 WP_000635249.1 peptide-binding protein -
QQS48_RS13530 (QQS48_13530) 83174..83800 - 627 Protein_100 DNA topoisomerase -
QQS48_RS13535 (QQS48_13535) 83876..84556 + 681 WP_010717201.1 IS6-like element IS1216 family transposase -
QQS48_RS13540 (QQS48_13540) 84590..85096 - 507 WP_002415429.1 trimethoprim-resistant dihydrofolate reductase DfrG -
QQS48_RS13545 (QQS48_13545) 85393..85710 - 318 WP_002326830.1 hypothetical protein -
QQS48_RS13550 (QQS48_13550) 86150..87013 - 864 WP_002332783.1 zeta toxin family protein Toxin
QQS48_RS13555 (QQS48_13555) 87015..87287 - 273 WP_002326825.1 antitoxin Antitoxin
QQS48_RS13560 (QQS48_13560) 87305..87520 - 216 WP_001835296.1 peptide-binding protein -
QQS48_RS13565 (QQS48_13565) 87612..88508 - 897 WP_002326827.1 ParA family protein -
QQS48_RS13570 (QQS48_13570) 88611..88871 - 261 Protein_108 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
QQS48_RS13575 (QQS48_13575) 89041..89778 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
QQS48_RS13580 (QQS48_13580) 89903..89986 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
QQS48_RS13585 (QQS48_13585) 90035..90118 - 84 Protein_111 MLS leader peptide -
QQS48_RS13590 (QQS48_13590) 90418..90789 - 372 WP_002358205.1 hypothetical protein -
QQS48_RS13595 (QQS48_13595) 90782..91627 - 846 WP_000239313.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid cat / tet(L) / tet(M) / erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) / aac(6')-aph(2'') / dfrG - 1..92098 92098
- inside IS/Tn erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) / aac(6')-aph(2'') / dfrG - 65370..89778 24408


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T283621 WP_002332783.1 NZ_CP127178:c87013-86150 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT283621 WP_002326825.1 NZ_CP127178:c87287-87015 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References