Detailed information of TA system TA028362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2188629..2189257
• Replicon: chromosome
• Accession: NC_018664
• Organism: Clostridium acidurici 9a

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K0B0M6
• Locus tag: CURI_RS10415
• Protein ID: WP_014968220.1
• Coordinates: 2188629..2188979 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K0B2C6
• Locus tag: CURI_RS10420
• Protein ID: WP_014968221.1
• Coordinates: 2188976..2189257 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CURI_RS10400	2183919..2184686	-	768	WP_014968217.1	WecB/TagA/CpsF family glycosyltransferase	-
CURI_RS10405	2184706..2185809	-	1104	WP_014968218.1	polysaccharide pyruvyl transferase CsaB	-
CURI_RS10410	2185802..2187961	-	2160	WP_014968219.1	hypothetical protein	-
CURI_RS10415	2188629..2188979	-	351	WP_014968220.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CURI_RS10420	2188976..2189257	-	282	WP_014968221.1	ribbon-helix-helix protein, CopG family	Antitoxin
CURI_RS10425	2189372..2190547	-	1176	WP_041701777.1	alanine racemase	-
CURI_RS10430	2190562..2191185	-	624	WP_014968223.1	outer membrane lipoprotein carrier protein LolA	-
CURI_RS10435	2191204..2192427	-	1224	WP_014968224.1	NAD(P)H-hydrate dehydratase	-
CURI_RS10440	2192501..2193124	-	624	WP_014968225.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12688.83 Da
• Isoelectric Point: 7.8515

>T28362 WP_014968220.1 NC_018664:c2188979-2188629 [Gottschalkia acidurici 9a]
VIVKRGDVFYADLSPVIGSEQGGVRPVLVVQNDIGNKYSPTIIISAITSQINKAKLPTHVEITAPEYGLPKDSVVLLEQI
RTIDKKRLREKIGHFDDGMMGKVDCCLKISIGLTDV

Nucleotide

• Length: 351 bp

>T28362 NC_018664:c2188979-2188629 [Gottschalkia acidurici 9a]
GTGATAGTAAAAAGAGGAGATGTTTTTTATGCAGACCTAAGTCCTGTTATTGGCTCAGAGCAGGGTGGTGTAAGACCTGT
ACTAGTTGTACAAAATGATATAGGTAATAAGTATAGCCCGACCATAATAATATCAGCAATTACATCTCAGATAAATAAAG
CTAAGTTGCCAACGCATGTAGAAATAACTGCACCAGAATATGGATTGCCTAAAGACTCAGTCGTATTACTTGAACAAATA
CGCACTATAGATAAGAAGCGATTGAGAGAAAAAATAGGACATTTTGATGATGGAATGATGGGAAAAGTAGATTGCTGTTT
AAAAATAAGCATAGGATTGACAGATGTCTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10816.58 Da
• Isoelectric Point: 4.5309

>AT28362 WP_014968221.1 NC_018664:c2189257-2188976 [Gottschalkia acidurici 9a]
MAETKKIIISLPNSLLEEVDDLVSIEEENRSEFIREAMKLYLRERKKIQVKESMKKGYLEMSQINVSLSEMGLAEDIREL
CIYETTLTGCEKL

Nucleotide

• Length: 282 bp

>AT28362 NC_018664:c2189257-2188976 [Gottschalkia acidurici 9a]
ATGGCCGAAACAAAAAAAATAATAATAAGTTTGCCCAACAGCTTGCTAGAAGAGGTAGACGATTTAGTGTCTATAGAAGA
GGAAAATAGAAGTGAATTTATACGAGAGGCCATGAAATTATATTTAAGAGAACGAAAGAAAATCCAGGTAAAGGAGAGTA
TGAAAAAGGGATATTTAGAAATGAGTCAAATAAATGTTTCTTTATCTGAAATGGGTCTAGCAGAAGATATAAGAGAGCTA
TGTATTTATGAGACAACTTTGACGGGGTGTGAGAAACTGTGA

Structures

Toxin

• AlphaFold DB: K0B0M6

Antitoxin

• AlphaFold DB: K0B2C6