Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 7714..8273 | Replicon | plasmid pAA-09EL71 |
| Accession | NC_018662 | ||
| Organism | Escherichia coli O104:H4 str. 2009EL-2071 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | G4VUF8 |
| Locus tag | O3O_RS26960 | Protein ID | WP_000421273.1 |
| Coordinates | 7714..7989 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0SRR3 |
| Locus tag | O3O_RS26965 | Protein ID | WP_001387467.1 |
| Coordinates | 7989..8273 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| O3O_RS26910 | 2756..2986 | + | 231 | WP_001261288.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | - |
| O3O_RS26915 | 3010..3411 | + | 402 | WP_001392130.1 | type II toxin-antitoxin system VapC family toxin | - |
| O3O_RS26920 | 3494..3643 | - | 150 | Protein_6 | transposase domain-containing protein | - |
| O3O_RS26935 | 4735..5712 | + | 978 | WP_001387465.1 | RepB family plasmid replication initiator protein | - |
| O3O_RS26940 | 5991..6731 | - | 741 | WP_001066951.1 | tyrosine-type recombinase/integrase | - |
| O3O_RS26945 | 6852..6977 | - | 126 | WP_032159539.1 | hypothetical protein | - |
| O3O_RS26950 | 7100..7261 | - | 162 | WP_001440647.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| O3O_RS26955 | 7377..7708 | - | 332 | Protein_11 | type II toxin-antitoxin system ParD family antitoxin | - |
| O3O_RS26960 | 7714..7989 | - | 276 | WP_000421273.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| O3O_RS26965 | 7989..8273 | - | 285 | WP_001387467.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| O3O_RS26970 | 9181..10009 | - | 829 | Protein_14 | incFII family plasmid replication initiator RepA | - |
| O3O_RS26980 | 10002..10076 | - | 75 | WP_014966221.1 | RepA leader peptide Tap | - |
| O3O_RS32030 | 10073..10207 | - | 135 | Protein_16 | protein CopA/IncA | - |
| O3O_RS26985 | 10314..10568 | - | 255 | WP_000083850.1 | replication regulatory protein RepA | - |
| O3O_RS26990 | 10849..11829 | + | 981 | WP_000019440.1 | IS5-like element ISKpn26 family transposase | - |
| O3O_RS26995 | 12280..12492 | - | 213 | Protein_19 | ANR family transcriptional regulator | - |
| O3O_RS27000 | 12557..13234 | + | 678 | WP_001339397.1 | IS66 family insertion sequence hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | aap/aspU / aggA / aggB / aggC / aggD | 1..75573 | 75573 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10765.45 Da Isoelectric Point: 9.9324
>T28361 WP_000421273.1 NC_018662:c7989-7714 [Escherichia coli O104:H4 str. 2009EL-2071]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T28361 NC_018662:c7989-7714 [Escherichia coli O104:H4 str. 2009EL-2071]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10460.87 Da Isoelectric Point: 5.6558
>AT28361 WP_001387467.1 NC_018662:c8273-7989 [Escherichia coli O104:H4 str. 2009EL-2071]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
Download Length: 285 bp
>AT28361 NC_018662:c8273-7989 [Escherichia coli O104:H4 str. 2009EL-2071]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y6H1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y880 |