Detailed information of TA system TA028360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 2756..3411
• Replicon: plasmid pAA-09EL71
• Accession: NC_018662
• Organism: Escherichia coli O104:H4 str. 2009EL-2071

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A2H3M3N3
• Locus tag: O3O_RS26915
• Protein ID: WP_001392130.1
• Coordinates: 3010..3411 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A0E0YAG4
• Locus tag: O3O_RS26910
• Protein ID: WP_001261288.1
• Coordinates: 2756..2986 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
O3O_RS26890	6..650	-	645	WP_001144032.1	ParA family protein	-
O3O_RS26895	829..1635	-	807	WP_000016961.1	site-specific integrase	-
O3O_RS26900	1636..1941	-	306	WP_001159872.1	type II toxin-antitoxin system toxin CcdB	-
O3O_RS26905	1943..2161	-	219	WP_000813638.1	type II toxin-antitoxin system antitoxin CcdA	-
O3O_RS26910	2756..2986	+	231	WP_001261288.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
O3O_RS26915	3010..3411	+	402	WP_001392130.1	type II toxin-antitoxin system VapC family toxin	Toxin
O3O_RS26920	3494..3643	-	150	Protein_6	transposase domain-containing protein	-
O3O_RS26935	4735..5712	+	978	WP_001387465.1	RepB family plasmid replication initiator protein	-
O3O_RS26940	5991..6731	-	741	WP_001066951.1	tyrosine-type recombinase/integrase	-
O3O_RS26945	6852..6977	-	126	WP_032159539.1	hypothetical protein	-
O3O_RS26950	7100..7261	-	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
O3O_RS26955	7377..7708	-	332	Protein_11	type II toxin-antitoxin system ParD family antitoxin	-
O3O_RS26960	7714..7989	-	276	WP_000421273.1	type II toxin-antitoxin system RelE/ParE family toxin	-
O3O_RS26965	7989..8273	-	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	aap/aspU / aggA / aggB / aggC / aggD	1..75573	75573

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14647.00 Da
• Isoelectric Point: 6.7123

>T28360 WP_001392130.1 NC_018662:3010-3411 [Escherichia coli O104:H4 str. 2009EL-2071]
MLDTNICSFIMREQPAAVIKRLEQAVLRNHRIVVSAITYAEMRFDATGPKASPRHIALVDAFCARLDAILPWDRAAVDAT
TEIKMALRLAETPIGPNNTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 402 bp

>T28360 NC_018662:3010-3411 [Escherichia coli O104:H4 str. 2009EL-2071]
ATGCTCGACACGAATATCTGCTCTTTCATTATGCGTGAACAGCCCGCCGCAGTGATCAAACGTCTGGAGCAGGCGGTGCT
GCGTAACCACCGCATTGTCGTGTCGGCCATCACCTATGCGGAGATGCGTTTCGACGCCACCGGCCCGAAAGCCTCACCGC
GTCATATTGCGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATTCTGCCCTGGGACCGCGCTGCCGTGGACGCCACC
ACGGAGATTAAAATGGCACTGCGGCTGGCCGAGACGCCAATCGGCCCGAACAACACGGCGATTGCCGGGCACGCCATCGC
TGCCGGGGCGATACTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCTGACCTGGTGCTGGAAGACTGGGTGAAGT
AA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8684.76 Da
• Isoelectric Point: 4.3654

>AT28360 WP_001261288.1 NC_018662:2756-2986 [Escherichia coli O104:H4 str. 2009EL-2071]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWSSFAALEKADADFMAEREDIVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT28360 NC_018662:2756-2986 [Escherichia coli O104:H4 str. 2009EL-2071]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTTGAGATCGTCCGCGAAGGAGACAGCATTATATTGCGCCCGGTCCGGCCGACCTGGAGCTCGTTTGCGGCACTCG
AAAAGGCCGACGCAGACTTTATGGCGGAGCGTGAGGACATTGTCAGCGACGAAGGAAGATTTGACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A2H3M3N3

Antitoxin

• AlphaFold DB: A0A0E0YAG4