Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1636..2161 Replicon plasmid pAA-09EL71
Accession NC_018662
Organism Escherichia coli O104:H4 str. 2009EL-2071

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0E0Y871
Locus tag O3O_RS26900 Protein ID WP_001159872.1
Coordinates 1636..1941 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0E0Y6P9
Locus tag O3O_RS26905 Protein ID WP_000813638.1
Coordinates 1943..2161 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3O_RS26890 6..650 - 645 WP_001144032.1 ParA family protein -
O3O_RS26895 829..1635 - 807 WP_000016961.1 site-specific integrase -
O3O_RS26900 1636..1941 - 306 WP_001159872.1 type II toxin-antitoxin system toxin CcdB Toxin
O3O_RS26905 1943..2161 - 219 WP_000813638.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
O3O_RS26910 2756..2986 + 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
O3O_RS26915 3010..3411 + 402 WP_001392130.1 type II toxin-antitoxin system VapC family toxin -
O3O_RS26920 3494..3643 - 150 Protein_6 transposase domain-containing protein -
O3O_RS26935 4735..5712 + 978 WP_001387465.1 RepB family plasmid replication initiator protein -
O3O_RS26940 5991..6731 - 741 WP_001066951.1 tyrosine-type recombinase/integrase -
O3O_RS26945 6852..6977 - 126 WP_032159539.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aap/aspU / aggA / aggB / aggC / aggD 1..75573 75573


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11722.51 Da        Isoelectric Point: 5.6919

>T28359 WP_001159872.1 NC_018662:c1941-1636 [Escherichia coli O104:H4 str. 2009EL-2071]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHWENDIKNAINLMFWGI

Download         Length: 306 bp

>T28359 NC_018662:c1941-1636 [Escherichia coli O104:H4 str. 2009EL-2071]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTAATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACTGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8344.29 Da        Isoelectric Point: 4.5203

>AT28359 WP_000813638.1 NC_018662:c2161-1943 [Escherichia coli O104:H4 str. 2009EL-2071]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVAKFIEMNGSFADENRDW

Download         Length: 219 bp

>AT28359 NC_018662:c2161-1943 [Escherichia coli O104:H4 str. 2009EL-2071]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAAGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y871

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6P9

References