Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5423981..5424606 Replicon chromosome
Accession NZ_CP127153
Organism Klebsiella pneumoniae subsp. pneumoniae strain T1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag QP513_RS27025 Protein ID WP_002882817.1
Coordinates 5423981..5424364 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag QP513_RS27030 Protein ID WP_004150355.1
Coordinates 5424364..5424606 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QP513_RS27010 (QP513_27010) 5421347..5422249 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
QP513_RS27015 (QP513_27015) 5422246..5422881 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
QP513_RS27020 (QP513_27020) 5422878..5423807 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
QP513_RS27025 (QP513_27025) 5423981..5424364 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
QP513_RS27030 (QP513_27030) 5424364..5424606 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
QP513_RS27035 (QP513_27035) 5424811..5425728 + 918 WP_002882812.1 alpha/beta hydrolase -
QP513_RS27040 (QP513_27040) 5425742..5426683 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
QP513_RS27045 (QP513_27045) 5426728..5427165 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
QP513_RS27050 (QP513_27050) 5427162..5428022 - 861 WP_002882807.1 virulence factor BrkB family protein -
QP513_RS27055 (QP513_27055) 5428016..5428615 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T283589 WP_002882817.1 NZ_CP127153:c5424364-5423981 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT283589 WP_004150355.1 NZ_CP127153:c5424606-5424364 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References