Detailed information of TA system TA283570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 141000..141505
• Replicon: chromosome
• Accession: NZ_CP127126
• Organism: Pseudomonas aeruginosa PA14 strain MA3 isolate DTU_MIE

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: P4A98_RS00645
• Protein ID: WP_003083773.1
• Coordinates: 141000..141281 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A0H2ZJU8
• Locus tag: P4A98_RS00650
• Protein ID: WP_003137009.1
• Coordinates: 141278..141505 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P4A98_RS00620 (P4A98_00620)	136251..137600	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
P4A98_RS00625 (P4A98_00625)	137649..138335	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
P4A98_RS00630 (P4A98_00630)	138436..139170	+	735	WP_003110658.1	GntR family transcriptional regulator	-
P4A98_RS00635 (P4A98_00635)	139350..139760	+	411	WP_003110659.1	aegerolysin family protein	-
P4A98_RS00640 (P4A98_00640)	139792..140700	-	909	WP_003083769.1	LysR family transcriptional regulator	-
P4A98_RS00645 (P4A98_00645)	141000..141281	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
P4A98_RS00650 (P4A98_00650)	141278..141505	-	228	WP_003137009.1	CopG family ribbon-helix-helix protein	Antitoxin
P4A98_RS00655 (P4A98_00655)	141681..142304	-	624	WP_003137011.1	hypothetical protein	-
P4A98_RS00660 (P4A98_00660)	142405..142905	+	501	WP_003137013.1	LEA type 2 family protein	-
P4A98_RS00665 (P4A98_00665)	142977..143318	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
P4A98_RS00670 (P4A98_00670)	143400..144827	-	1428	WP_003137015.1	GABA permease	-
P4A98_RS00675 (P4A98_00675)	144996..146489	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T283570 WP_003083773.1 NZ_CP127126:c141281-141000 [Pseudomonas aeruginosa PA14]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T283570 NZ_CP127126:c141281-141000 [Pseudomonas aeruginosa PA14]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8526.56 Da
• Isoelectric Point: 5.5740

>AT283570 WP_003137009.1 NZ_CP127126:c141505-141278 [Pseudomonas aeruginosa PA14]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHAEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT283570 NZ_CP127126:c141505-141278 [Pseudomonas aeruginosa PA14]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAGCACGCCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A0H2ZJU8