Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4834108..4834605 Replicon chromosome
Accession NC_018661
Organism Escherichia coli O104:H4 str. 2009EL-2071

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag O3O_RS24550 Protein ID WP_001173343.1
Coordinates 4834108..4834281 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag O3O_RS24555 Protein ID WP_000398619.1
Coordinates 4834309..4834605 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3O_RS24540 4830778..4832235 + 1458 WP_000856837.1 dipeptide/tripeptide permease DtpC -
O3O_RS24545 4832472..4833989 + 1518 WP_001295074.1 lysine--tRNA ligase -
O3O_RS24550 4834108..4834281 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
O3O_RS24555 4834309..4834605 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
O3O_RS24565 4834832..4835104 - 273 WP_000405647.1 N-acetyltransferase -
O3O_RS24570 4835116..4835346 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
O3O_RS24575 4835527..4837158 + 1632 WP_001216486.1 sensor histidine kinase -
O3O_RS24580 4837155..4837874 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T28357 WP_001173343.1 NC_018661:c4834281-4834108 [Escherichia coli O104:H4 str. 2009EL-2071]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T28357 NC_018661:c4834281-4834108 [Escherichia coli O104:H4 str. 2009EL-2071]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT28357 WP_000398619.1 NC_018661:c4834605-4834309 [Escherichia coli O104:H4 str. 2009EL-2071]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT28357 NC_018661:c4834605-4834309 [Escherichia coli O104:H4 str. 2009EL-2071]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References