Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2479700..2480336 Replicon chromosome
Accession NZ_CP127095
Organism Bacillus inaquosorum strain LBA001

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QMC72_RS12890 Protein ID WP_003156187.1
Coordinates 2479700..2480050 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag QMC72_RS12895 Protein ID WP_003225183.1
Coordinates 2480055..2480336 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMC72_RS12850 (QMC72_12850) 2474744..2475343 - 600 WP_133488401.1 phosphoserine phosphatase RsbX -
QMC72_RS12855 (QMC72_12855) 2475343..2476131 - 789 WP_003240361.1 RNA polymerase sigma factor SigB -
QMC72_RS12860 (QMC72_12860) 2476097..2476579 - 483 WP_003240359.1 anti-sigma B factor RsbW -
QMC72_RS12865 (QMC72_12865) 2476576..2476905 - 330 WP_003240357.1 anti-sigma factor antagonist RsbV -
QMC72_RS12870 (QMC72_12870) 2476967..2477974 - 1008 WP_003240354.1 phosphoserine phosphatase RsbU -
QMC72_RS12875 (QMC72_12875) 2477986..2478387 - 402 WP_003240352.1 serine/threonine-protein kinase RsbT -
QMC72_RS12880 (QMC72_12880) 2478391..2478756 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
QMC72_RS12885 (QMC72_12885) 2478761..2479585 - 825 WP_133488403.1 RsbT co-antagonist protein RsbRA -
QMC72_RS12890 (QMC72_12890) 2479700..2480050 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QMC72_RS12895 (QMC72_12895) 2480055..2480336 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QMC72_RS12900 (QMC72_12900) 2480451..2481620 - 1170 WP_133488405.1 alanine racemase -
QMC72_RS12905 (QMC72_12905) 2481735..2482751 - 1017 WP_133488407.1 outer membrane lipoprotein carrier protein LolA -
QMC72_RS12910 (QMC72_12910) 2482916..2483281 - 366 WP_003240339.1 holo-ACP synthase -
QMC72_RS12915 (QMC72_12915) 2483376..2483975 + 600 WP_003240338.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T283553 WP_003156187.1 NZ_CP127095:c2480050-2479700 [Bacillus inaquosorum]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT283553 WP_003225183.1 NZ_CP127095:c2480336-2480055 [Bacillus inaquosorum]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References