Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4686008..4686603 Replicon chromosome
Accession NC_018661
Organism Escherichia coli O104:H4 str. 2009EL-2071

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag O3O_RS23755 Protein ID WP_000239579.1
Coordinates 4686008..4686358 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag O3O_RS23760 Protein ID WP_001223208.1
Coordinates 4686352..4686603 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3O_RS23735 4681284..4682306 - 1023 WP_001387274.1 ABC transporter permease -
O3O_RS23740 4682320..4683822 - 1503 WP_000205794.1 sugar ABC transporter ATP-binding protein -
O3O_RS23745 4684132..4685088 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
O3O_RS23750 4685398..4685928 + 531 WP_000055072.1 inorganic diphosphatase -
O3O_RS23755 4686008..4686358 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
O3O_RS23760 4686352..4686603 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
O3O_RS23765 4686815..4687156 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
O3O_RS23770 4687159..4690938 - 3780 WP_000060946.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T28355 WP_000239579.1 NC_018661:c4686358-4686008 [Escherichia coli O104:H4 str. 2009EL-2071]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T28355 NC_018661:c4686358-4686008 [Escherichia coli O104:H4 str. 2009EL-2071]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT28355 WP_001223208.1 NC_018661:c4686603-4686352 [Escherichia coli O104:H4 str. 2009EL-2071]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT28355 NC_018661:c4686603-4686352 [Escherichia coli O104:H4 str. 2009EL-2071]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References