Detailed information of TA system TA028350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3095123..3095604
• Replicon: chromosome
• Accession: NC_018661
• Organism: Escherichia coli O104:H4 str. 2009EL-2071

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7YS45
• Locus tag: O3O_RS15800
• Protein ID: WP_014966210.1
• Coordinates: 3095314..3095604 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A0D7Y007
• Locus tag: O3O_RS15795
• Protein ID: WP_000100896.1
• Coordinates: 3095123..3095314 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
O3O_RS31610	3090275..3090453	-	179	Protein_2993	hypothetical protein	-
O3O_RS15755	3090455..3091204	-	750	WP_001289900.1	ead/Ea22-like family protein	-
O3O_RS15760	3091201..3091887	-	687	WP_000034251.1	ead/Ea22-like family protein	-
O3O_RS15765	3091874..3092368	-	495	WP_001275731.1	class I SAM-dependent methyltransferase	-
O3O_RS15770	3092365..3092787	-	423	WP_000416574.1	DUF977 family protein	-
O3O_RS15775	3092828..3093790	-	963	WP_000095671.1	helix-turn-helix domain-containing protein	-
O3O_RS15780	3093813..3094238	-	426	WP_000693845.1	Rha family transcriptional regulator	-
O3O_RS15785	3094222..3094497	-	276	WP_001048458.1	helix-turn-helix domain-containing protein	-
O3O_RS15790	3094605..3095105	+	501	WP_000824162.1	helix-turn-helix transcriptional regulator	-
O3O_RS15795	3095123..3095314	+	192	WP_000100896.1	hypothetical protein	Antitoxin
O3O_RS15800	3095314..3095604	+	291	WP_014966210.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
O3O_RS15805	3095874..3096026	+	153	WP_000379548.1	DUF1391 family protein	-
O3O_RS15810	3096038..3096376	+	339	WP_000394541.1	hypothetical protein	-
O3O_RS15815	3096365..3096631	-	267	WP_012601410.1	hypothetical protein	-
O3O_RS15820	3097126..3097314	+	189	WP_000449175.1	cell division inhibitor	-
O3O_RS15825	3097311..3097499	+	189	WP_000199475.1	DUF1482 family protein	-
O3O_RS15830	3097592..3100030	+	2439	WP_000102130.1	exonuclease	-
O3O_RS15835	3100092..3100361	+	270	WP_000003742.1	excisionase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3051039..3108617	57578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11176.05 Da
• Isoelectric Point: 9.9854

>T28350 WP_014966210.1 NC_018661:3095314-3095604 [Escherichia coli O104:H4 str. 2009EL-2071]
MLPILWLPSARDDLRQIVAYIAKENPPAARRLKIRIETSVLSLTEHPYLYPPSERVPSLREIVTHPNYIILYRVTASNIE
IVNVVHSRRQYPNKTC

Nucleotide

• Length: 291 bp

>T28350 NC_018661:3095314-3095604 [Escherichia coli O104:H4 str. 2009EL-2071]
ATGCTGCCCATTTTATGGCTACCTTCTGCACGTGATGATTTACGTCAGATCGTAGCCTATATTGCTAAGGAAAACCCTCC
CGCTGCACGTAGACTAAAAATACGCATTGAAACATCAGTTTTGTCACTTACTGAACACCCTTATCTGTACCCACCGAGCG
AAAGAGTTCCAAGTCTTCGTGAGATAGTGACTCATCCTAACTACATAATACTTTACCGAGTAACAGCATCTAACATCGAG
ATCGTAAATGTAGTTCACTCACGAAGACAGTATCCAAACAAAACCTGTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7033.83 Da
• Isoelectric Point: 4.0270

>AT28350 WP_000100896.1 NC_018661:3095123-3095314 [Escherichia coli O104:H4 str. 2009EL-2071]
MSTALSPIISEFETVEQENSYNEWLRTKVAASLSDPRPAIPHDEVMAEMENLIAQLAATNRSE

Nucleotide

• Length: 192 bp

>AT28350 NC_018661:3095123-3095314 [Escherichia coli O104:H4 str. 2009EL-2071]
ATGAGTACAGCCCTTTCCCCGATAATTTCTGAATTTGAAACAGTCGAACAAGAAAACAGCTATAACGAATGGTTGCGAAC
CAAAGTGGCAGCAAGCCTCTCAGATCCCCGTCCTGCAATTCCACATGACGAAGTAATGGCTGAAATGGAAAACCTTATTG
CTCAATTAGCTGCAACGAACAGGAGTGAGTAA

Structures

Toxin

• AlphaFold DB: A0A0D7YS45

Antitoxin

• AlphaFold DB: A0A0D7Y007