Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 328651..329288 Replicon chromosome
Accession NZ_CP126986
Organism Bacillus velezensis strain MC2S-6

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QQF37_RS01560 Protein ID WP_003156187.1
Coordinates 328938..329288 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag QQF37_RS01555 Protein ID WP_003156188.1
Coordinates 328651..328932 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQF37_RS01535 (QQF37_01535) 325016..325615 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
QQF37_RS01540 (QQF37_01540) 325708..326073 + 366 WP_007609580.1 holo-ACP synthase -
QQF37_RS01545 (QQF37_01545) 326238..327245 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
QQF37_RS01550 (QQF37_01550) 327362..328531 + 1170 WP_032873001.1 alanine racemase -
QQF37_RS01555 (QQF37_01555) 328651..328932 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QQF37_RS01560 (QQF37_01560) 328938..329288 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QQF37_RS01565 (QQF37_01565) 329406..330227 + 822 WP_003156182.1 STAS domain-containing protein -
QQF37_RS01570 (QQF37_01570) 330232..330597 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
QQF37_RS01575 (QQF37_01575) 330600..331001 + 402 WP_003156178.1 anti-sigma regulatory factor -
QQF37_RS01580 (QQF37_01580) 331013..332020 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
QQF37_RS01585 (QQF37_01585) 332084..332413 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
QQF37_RS01590 (QQF37_01590) 332410..332892 + 483 WP_007609591.1 anti-sigma B factor RsbW -
QQF37_RS01595 (QQF37_01595) 332858..333646 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
QQF37_RS01600 (QQF37_01600) 333646..334248 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T283448 WP_003156187.1 NZ_CP126986:328938-329288 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT283448 WP_003156188.1 NZ_CP126986:328651-328932 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References