Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 16169..16794 Replicon plasmid pEND_Eco 12049-1
Accession NZ_CP126953
Organism Escherichia coli O78:H4 strain APEC E12049

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag QQA22_RS25245 Protein ID WP_000911333.1
Coordinates 16169..16567 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID V0VCB2
Locus tag QQA22_RS25250 Protein ID WP_000450520.1
Coordinates 16567..16794 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA22_RS25230 (12500) 12500..13009 + 510 WP_000628107.1 conjugal transfer entry exclusion protein TraS -
QQA22_RS25235 (13023) 13023..13754 + 732 WP_000850422.1 conjugal transfer complement resistance protein TraT -
QQA22_RS25240 (14007) 14007..16160 + 2154 WP_000009325.1 type IV conjugative transfer system coupling protein TraD -
QQA22_RS25245 (16169) 16169..16567 - 399 WP_000911333.1 tRNA(fMet)-specific endonuclease VapC Toxin
QQA22_RS25250 (16567) 16567..16794 - 228 WP_000450520.1 toxin-antitoxin system antitoxin VapB Antitoxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iroB / iroC / iroD / iroE / iroN / iucA / iucB / iucC / iucD / iutA 1..155857 155857


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(8-50)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14919.19 Da        Isoelectric Point: 7.8605

>T283359 WP_000911333.1 NZ_CP126953:c16567-16169 [Escherichia coli O78:H4]
MLKFMLDTNICIFTMKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Download         Length: 399 bp

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8596.63 Da        Isoelectric Point: 4.7513

>AT283359 WP_000450520.1 NZ_CP126953:c16794-16567 [Escherichia coli O78:H4]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Download         Length: 228 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB V0VCB2

References