Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4648410..4649005 Replicon chromosome
Accession NC_018658
Organism Escherichia coli O104:H4 str. 2011C-3493

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag O3K_RS22690 Protein ID WP_000239579.1
Coordinates 4648410..4648760 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag O3K_RS22695 Protein ID WP_001223208.1
Coordinates 4648754..4649005 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3K_RS22670 4643686..4644708 - 1023 WP_001387274.1 ABC transporter permease -
O3K_RS22675 4644722..4646224 - 1503 WP_000205794.1 sugar ABC transporter ATP-binding protein -
O3K_RS22680 4646534..4647490 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
O3K_RS22685 4647800..4648330 + 531 WP_000055072.1 inorganic diphosphatase -
O3K_RS22690 4648410..4648760 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
O3K_RS22695 4648754..4649005 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
O3K_RS22700 4649217..4649558 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
O3K_RS22705 4649561..4653340 - 3780 WP_000060946.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T28326 WP_000239579.1 NC_018658:c4648760-4648410 [Escherichia coli O104:H4 str. 2011C-3493]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T28326 NC_018658:c4648760-4648410 [Escherichia coli O104:H4 str. 2011C-3493]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT28326 WP_001223208.1 NC_018658:c4649005-4648754 [Escherichia coli O104:H4 str. 2011C-3493]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT28326 NC_018658:c4649005-4648754 [Escherichia coli O104:H4 str. 2011C-3493]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References