Detailed information of TA system TA028324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3969653..3970490
• Replicon: chromosome
• Accession: NC_018658
• Organism: Escherichia coli O104:H4 str. 2011C-3493

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: O3K_RS19505
• Protein ID: WP_000227784.1
• Coordinates: 3969948..3970490 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: O3K_RS19500
• Protein ID: WP_001297137.1
• Coordinates: 3969653..3969964 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
O3K_RS19475	3964673..3965620	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
O3K_RS19480	3965642..3967633	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
O3K_RS19485	3967623..3968237	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
O3K_RS19490	3968237..3968566	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
O3K_RS19495	3968578..3969468	+	891	WP_000971336.1	heme o synthase	-
O3K_RS19500	3969653..3969964	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
O3K_RS19505	3969948..3970490	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
O3K_RS19510	3970546..3971481	-	936	WP_024174603.1	sel1 repeat family protein	-
O3K_RS19515	3971889..3973253	+	1365	WP_001000974.1	MFS transporter	-
O3K_RS19520	3973381..3973872	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
O3K_RS19525	3974040..3974951	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T28324 WP_000227784.1 NC_018658:3969948-3970490 [Escherichia coli O104:H4 str. 2011C-3493]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T28324 NC_018658:3969948-3970490 [Escherichia coli O104:H4 str. 2011C-3493]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT28324 WP_001297137.1 NC_018658:3969653-3969964 [Escherichia coli O104:H4 str. 2011C-3493]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT28324 NC_018658:3969653-3969964 [Escherichia coli O104:H4 str. 2011C-3493]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3