Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 19730..20373 Replicon plasmid pEND_Eco 18055
Accession NZ_CP126941
Organism Escherichia coli O2:K1:H7 strain APEC E18055

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag QQA30_RS25445 Protein ID WP_001034044.1
Coordinates 19957..20373 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag QQA30_RS25440 Protein ID WP_001261286.1
Coordinates 19730..19960 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA30_RS25420 (14885) 14885..15310 - 426 WP_021514830.1 transposase -
QQA30_RS25425 (15397) 15397..15510 + 114 Protein_17 VapC toxin family PIN domain ribonuclease -
QQA30_RS25430 (15555) 15555..18767 - 3213 WP_289251513.1 pcar -
QQA30_RS25435 (18855) 18855..19379 - 525 WP_289251514.1 hypothetical protein -
QQA30_RS25440 (19730) 19730..19960 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QQA30_RS25445 (19957) 19957..20373 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
QQA30_RS25450 (20448) 20448..22013 + 1566 WP_001128474.1 AAA family ATPase -
QQA30_RS25455 (21998) 21998..23020 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat 1..199896 199896
- flank IS/Tn - - 23274..23777 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T283189 WP_001034044.1 NZ_CP126941:19957-20373 [Escherichia coli O2:K1:H7]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT283189 WP_001261286.1 NZ_CP126941:19730-19960 [Escherichia coli O2:K1:H7]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References