Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3322910..3323710 Replicon chromosome
Accession NZ_CP126934
Organism Escherichia coli O78:H4 strain APEC E19057

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag QQA26_RS16640 Protein ID WP_000342449.1
Coordinates 3322910..3323437 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag QQA26_RS16645 Protein ID WP_001277108.1
Coordinates 3323444..3323710 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA26_RS16615 (3317985) 3317985..3318752 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
QQA26_RS16620 (3318749) 3318749..3320026 - 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
QQA26_RS16625 (3320023) 3320023..3320949 - 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
QQA26_RS16630 (3320997) 3320997..3322106 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
QQA26_RS16635 (3322530) 3322530..3322913 + 384 WP_000778777.1 aspartate 1-decarboxylase autocleavage activator PanM -
QQA26_RS16640 (3322910) 3322910..3323437 - 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
QQA26_RS16645 (3323444) 3323444..3323710 - 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
QQA26_RS16650 (3323860) 3323860..3324963 - 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
QQA26_RS16655 (3325235) 3325235..3326089 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
QQA26_RS16660 (3326334) 3326334..3327392 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
QQA26_RS16665 (3327385) 3327385..3328053 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T283126 WP_000342449.1 NZ_CP126934:c3323437-3322910 [Escherichia coli O78:H4]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT283126 WP_001277108.1 NZ_CP126934:c3323710-3323444 [Escherichia coli O78:H4]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References