Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1462059..1462684 Replicon chromosome
Accession NC_018658
Organism Escherichia coli O104:H4 str. 2011C-3493

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag O3K_RS07065 Protein ID WP_000911329.1
Coordinates 1462286..1462684 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag O3K_RS07060 Protein ID WP_000450524.1
Coordinates 1462059..1462286 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3K_RS07035 1457861..1458331 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
O3K_RS07040 1458331..1458903 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
O3K_RS07045 1459049..1459927 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
O3K_RS07050 1459944..1460978 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
O3K_RS07055 1461191..1461904 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
O3K_RS07060 1462059..1462286 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
O3K_RS07065 1462286..1462684 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
O3K_RS07070 1462831..1463694 + 864 WP_001267498.1 neutral zinc metallopeptidase -
O3K_RS07075 1463709..1465724 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
O3K_RS07080 1465798..1466496 + 699 WP_000679823.1 esterase -
O3K_RS07085 1466606..1466806 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T28311 WP_000911329.1 NC_018658:1462286-1462684 [Escherichia coli O104:H4 str. 2011C-3493]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T28311 NC_018658:1462286-1462684 [Escherichia coli O104:H4 str. 2011C-3493]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT28311 WP_000450524.1 NC_018658:1462059-1462286 [Escherichia coli O104:H4 str. 2011C-3493]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT28311 NC_018658:1462059-1462286 [Escherichia coli O104:H4 str. 2011C-3493]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References