Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1017922..1018576 Replicon chromosome
Accession NC_018658
Organism Escherichia coli O104:H4 str. 2011C-3493

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag O3K_RS04960 Protein ID WP_000244772.1
Coordinates 1018169..1018576 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag O3K_RS04955 Protein ID WP_000354046.1
Coordinates 1017922..1018188 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3K_RS04930 1013209..1013952 + 744 WP_000951964.1 SDR family oxidoreductase -
O3K_RS04935 1014009..1015442 - 1434 WP_001387034.1 6-phospho-beta-glucosidase BglA -
O3K_RS04940 1015487..1015798 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
O3K_RS04945 1015962..1016621 + 660 WP_000250274.1 hemolysin III family protein -
O3K_RS04950 1016699..1017679 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
O3K_RS04955 1017922..1018188 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
O3K_RS04960 1018169..1018576 + 408 WP_000244772.1 protein YgfX Toxin
O3K_RS04965 1018616..1019137 - 522 WP_001055874.1 flavodoxin FldB -
O3K_RS04970 1019249..1020145 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
O3K_RS04975 1020170..1020880 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
O3K_RS04980 1020886..1022619 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T28309 WP_000244772.1 NC_018658:1018169-1018576 [Escherichia coli O104:H4 str. 2011C-3493]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T28309 NC_018658:1018169-1018576 [Escherichia coli O104:H4 str. 2011C-3493]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT28309 WP_000354046.1 NC_018658:1017922-1018188 [Escherichia coli O104:H4 str. 2011C-3493]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT28309 NC_018658:1017922-1018188 [Escherichia coli O104:H4 str. 2011C-3493]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References