Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1108043..1108626 Replicon chromosome
Accession NZ_CP126931
Organism Escherichia coli O78:H51 strain APEC E19025

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag QQA25_RS05380 Protein ID WP_000254745.1
Coordinates 1108291..1108626 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag QQA25_RS05375 Protein ID WP_000581937.1
Coordinates 1108043..1108291 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA25_RS05365 (1104382) 1104382..1105683 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
QQA25_RS05370 (1105731) 1105731..1107965 + 2235 WP_000226815.1 GTP pyrophosphokinase -
QQA25_RS05375 (1108043) 1108043..1108291 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
QQA25_RS05380 (1108291) 1108291..1108626 + 336 WP_000254745.1 endoribonuclease MazF Toxin
QQA25_RS05385 (1108697) 1108697..1109488 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
QQA25_RS05390 (1109716) 1109716..1111353 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
QQA25_RS05395 (1111441) 1111441..1112739 + 1299 WP_000036723.1 phosphopyruvate hydratase -
QQA25_RS05400 (1112795) 1112795..1113157 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T283084 WP_000254745.1 NZ_CP126931:1108291-1108626 [Escherichia coli O78:H51]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT283084 WP_000581937.1 NZ_CP126931:1108043-1108291 [Escherichia coli O78:H51]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References