Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 41749..42392 Replicon plasmid pEND_Eco 12053-1
Accession NZ_CP126929
Organism Escherichia coli O2:K1:H4 strain APEC E12053

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag QQA27_RS24170 Protein ID WP_001034044.1
Coordinates 41976..42392 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag QQA27_RS24165 Protein ID WP_001261286.1
Coordinates 41749..41979 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA27_RS24150 (36886) 36886..37116 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
QQA27_RS24155 (37113) 37113..37529 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
QQA27_RS24160 (37574) 37574..41368 - 3795 WP_001144732.1 hypothetical protein -
QQA27_RS24165 (41749) 41749..41979 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QQA27_RS24170 (41976) 41976..42392 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
QQA27_RS24175 (42467) 42467..44032 + 1566 WP_001128474.1 AAA family ATPase -
QQA27_RS24180 (44017) 44017..45039 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat 1..189727 189727
- flank IS/Tn - - 45293..45793 500


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T283074 WP_001034044.1 NZ_CP126929:41976-42392 [Escherichia coli O2:K1:H4]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT283074 WP_001261286.1 NZ_CP126929:41749-41979 [Escherichia coli O2:K1:H4]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References