Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4003..4528 Replicon plasmid pEND_Eco 19035-1
Accession NZ_CP126927
Organism Escherichia coli O2:K1:H5 strain APEC E19035

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag QQA32_RS26135 Protein ID WP_001159871.1
Coordinates 4003..4308 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag QQA32_RS26140 Protein ID WP_000813630.1
Coordinates 4310..4528 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA32_RS26125 (1815) 1815..2516 + 702 WP_103433257.1 replication initiation protein -
QQA32_RS26130 (3196) 3196..4002 - 807 WP_021512277.1 site-specific integrase -
QQA32_RS26135 (4003) 4003..4308 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
QQA32_RS26140 (4310) 4310..4528 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
QQA32_RS26145 (5114) 5114..5344 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
QQA32_RS26150 (5341) 5341..5757 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat 1..193604 193604


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T283034 WP_001159871.1 NZ_CP126927:c4308-4003 [Escherichia coli O2:K1:H5]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT283034 WP_000813630.1 NZ_CP126927:c4528-4310 [Escherichia coli O2:K1:H5]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References