Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1188580..1189163 Replicon chromosome
Accession NZ_CP126926
Organism Escherichia coli O2:K1:H5 strain APEC E19035

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag QQA32_RS05740 Protein ID WP_000254750.1
Coordinates 1188828..1189163 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag QQA32_RS05735 Protein ID WP_000581937.1
Coordinates 1188580..1188828 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA32_RS05725 (1184919) 1184919..1186220 + 1302 WP_000046816.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
QQA32_RS05730 (1186268) 1186268..1188502 + 2235 WP_000226815.1 GTP pyrophosphokinase -
QQA32_RS05735 (1188580) 1188580..1188828 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
QQA32_RS05740 (1188828) 1188828..1189163 + 336 WP_000254750.1 endoribonuclease MazF Toxin
QQA32_RS05745 (1189235) 1189235..1190026 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
QQA32_RS05750 (1190254) 1190254..1191891 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
QQA32_RS05755 (1191979) 1191979..1193277 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T283020 WP_000254750.1 NZ_CP126926:1188828-1189163 [Escherichia coli O2:K1:H5]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT283020 WP_000581937.1 NZ_CP126926:1188580-1188828 [Escherichia coli O2:K1:H5]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References