Detailed information of TA system TA028298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3960309..3961146
• Replicon: chromosome
• Accession: NC_018650
• Organism: Escherichia coli O104:H4 str. 2009EL-2050

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: O3M_RS20150
• Protein ID: WP_000227784.1
• Coordinates: 3960604..3961146 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: O3M_RS20145
• Protein ID: WP_001297137.1
• Coordinates: 3960309..3960620 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
O3M_RS20120	3955329..3956276	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
O3M_RS20125	3956298..3958289	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
O3M_RS20130	3958279..3958893	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
O3M_RS20135	3958893..3959222	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
O3M_RS20140	3959234..3960124	+	891	WP_000971336.1	heme o synthase	-
O3M_RS20145	3960309..3960620	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
O3M_RS20150	3960604..3961146	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
O3M_RS20155	3961202..3962137	-	936	WP_024174603.1	sel1 repeat family protein	-
O3M_RS20160	3962545..3963909	+	1365	WP_001000974.1	MFS transporter	-
O3M_RS20165	3964037..3964528	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
O3M_RS20170	3964696..3965607	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T28298 WP_000227784.1 NC_018650:3960604-3961146 [Escherichia coli O104:H4 str. 2009EL-2050]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T28298 NC_018650:3960604-3961146 [Escherichia coli O104:H4 str. 2009EL-2050]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT28298 WP_001297137.1 NC_018650:3960309-3960620 [Escherichia coli O104:H4 str. 2009EL-2050]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT28298 NC_018650:3960309-3960620 [Escherichia coli O104:H4 str. 2009EL-2050]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3