Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3926232..3926850 Replicon chromosome
Accession NC_018650
Organism Escherichia coli O104:H4 str. 2009EL-2050

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag O3M_RS19980 Protein ID WP_001291435.1
Coordinates 3926632..3926850 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag O3M_RS19975 Protein ID WP_000344800.1
Coordinates 3926232..3926606 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3M_RS19965 3921321..3922514 + 1194 WP_001386618.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
O3M_RS19970 3922537..3925686 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
O3M_RS19975 3926232..3926606 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
O3M_RS19980 3926632..3926850 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
O3M_RS19985 3927021..3927572 + 552 WP_000102564.1 maltose O-acetyltransferase -
O3M_RS19990 3927688..3928158 + 471 WP_000136192.1 YlaC family protein -
O3M_RS19995 3928322..3929872 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
O3M_RS20000 3929914..3930267 - 354 WP_000878140.1 DUF1428 domain-containing protein -
O3M_RS20005 3930646..3930957 + 312 WP_000409911.1 MGMT family protein -
O3M_RS20010 3930988..3931560 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T28297 WP_001291435.1 NC_018650:3926632-3926850 [Escherichia coli O104:H4 str. 2009EL-2050]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T28297 NC_018650:3926632-3926850 [Escherichia coli O104:H4 str. 2009EL-2050]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT28297 WP_000344800.1 NC_018650:3926232-3926606 [Escherichia coli O104:H4 str. 2009EL-2050]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT28297 NC_018650:3926232-3926606 [Escherichia coli O104:H4 str. 2009EL-2050]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References