Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 51468..52271 Replicon plasmid pblaNDM5-S1
Accession NZ_CP126537
Organism Citrobacter freundii strain CRNMS1

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag QOK75_RS26760 Protein ID WP_022652313.1
Coordinates 51741..52271 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag QOK75_RS26755 Protein ID WP_022652312.1
Coordinates 51468..51737 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QOK75_RS26740 (QOK75_26740) 47773..48080 - 308 Protein_66 IS1-like element transposase -
QOK75_RS26745 (QOK75_26745) 48557..49297 + 741 WP_022652310.1 site-specific integrase -
QOK75_RS26750 (QOK75_26750) 49623..50612 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
QOK75_RS26755 (QOK75_26755) 51468..51737 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
QOK75_RS26760 (QOK75_26760) 51741..52271 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
QOK75_RS26765 (QOK75_26765) 52439..53296 + 858 WP_043002029.1 IS5 family transposase -
QOK75_RS26770 (QOK75_26770) 53437..54330 - 894 Protein_72 IS3 family transposase -
QOK75_RS26775 (QOK75_26775) 54398..55549 + 1152 WP_022652315.1 IS30-like element IS30 family transposase -
QOK75_RS26780 (QOK75_26780) 55658..56041 + 384 WP_001201739.1 transposase -
QOK75_RS26785 (QOK75_26785) 56038..56385 + 348 WP_000609174.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / floR / aadA5 / qacE / sul1 / qnrA1 / blaNDM-5 - 1..197210 197210
- inside IScluster/Tn - - 38525..62140 23615


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T282956 WP_022652313.1 NZ_CP126537:51741-52271 [Citrobacter freundii]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT282956 WP_022652312.1 NZ_CP126537:51468-51737 [Citrobacter freundii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References