Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5145620..5146236 Replicon chromosome
Accession NZ_CP126536
Organism Citrobacter freundii strain CRNMS1

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag QOK75_RS25565 Protein ID WP_003028682.1
Coordinates 5145620..5145994 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag QOK75_RS25570 Protein ID WP_043018956.1
Coordinates 5145994..5146236 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QOK75_RS25550 (5143123) 5143123..5144025 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
QOK75_RS25555 (5144022) 5144022..5144657 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
QOK75_RS25560 (5144654) 5144654..5145583 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
QOK75_RS25565 (5145620) 5145620..5145994 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
QOK75_RS25570 (5145994) 5145994..5146236 - 243 WP_043018956.1 CopG family transcriptional regulator Antitoxin
QOK75_RS25575 (5146442) 5146442..5147350 + 909 WP_003847901.1 alpha/beta hydrolase -
QOK75_RS25580 (5147501) 5147501..5148442 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
QOK75_RS25585 (5148487) 5148487..5148924 - 438 WP_003028671.1 D-aminoacyl-tRNA deacylase -
QOK75_RS25590 (5148921) 5148921..5149793 - 873 WP_003028669.1 virulence factor BrkB family protein -
QOK75_RS25595 (5149787) 5149787..5150386 - 600 WP_003028663.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T282954 WP_003028682.1 NZ_CP126536:c5145994-5145620 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT282954 WP_043018956.1 NZ_CP126536:c5146236-5145994 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References