Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 290591..291391 Replicon chromosome
Accession NZ_CP126348
Organism Escherichia coli strain NN5

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag QN227_RS01330 Protein ID WP_000342449.1
Coordinates 290864..291391 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag QN227_RS01325 Protein ID WP_001277108.1
Coordinates 290591..290857 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QN227_RS01305 (286249) 286249..286917 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
QN227_RS01310 (286910) 286910..287968 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
QN227_RS01315 (288213) 288213..289067 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
QN227_RS01320 (289338) 289338..290441 + 1104 WP_077778735.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
QN227_RS01325 (290591) 290591..290857 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
QN227_RS01330 (290864) 290864..291391 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
QN227_RS01335 (291388) 291388..291771 - 384 WP_033557539.1 aspartate 1-decarboxylase autocleavage activator PanM -
QN227_RS01340 (292195) 292195..293304 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
QN227_RS01345 (293352) 293352..294278 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
QN227_RS01350 (294275) 294275..295552 + 1278 WP_033557542.1 branched chain amino acid ABC transporter permease LivM -
QN227_RS01355 (295549) 295549..296316 + 768 WP_000082110.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T282892 WP_000342449.1 NZ_CP126348:290864-291391 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT282892 WP_001277108.1 NZ_CP126348:290591-290857 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References