Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3539008..3539845 Replicon chromosome
Accession NZ_CP126345
Organism Escherichia coli strain DX6

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag QN223_RS17375 Protein ID WP_000227784.1
Coordinates 3539303..3539845 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag QN223_RS17370 Protein ID WP_001297137.1
Coordinates 3539008..3539319 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QN223_RS17345 (3534028) 3534028..3534975 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
QN223_RS17350 (3534997) 3534997..3536988 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
QN223_RS17355 (3536978) 3536978..3537592 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
QN223_RS17360 (3537592) 3537592..3537921 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
QN223_RS17365 (3537933) 3537933..3538823 + 891 WP_000971336.1 heme o synthase -
QN223_RS17370 (3539008) 3539008..3539319 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
QN223_RS17375 (3539303) 3539303..3539845 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
QN223_RS17380 (3539901) 3539901..3540836 - 936 WP_001297127.1 tetratricopeptide repeat protein -
QN223_RS17385 (3541244) 3541244..3542608 + 1365 WP_001000978.1 MFS transporter -
QN223_RS17390 (3542736) 3542736..3543227 - 492 WP_001138904.1 nucleotide binding protein YajQ -
QN223_RS17395 (3543395) 3543395..3544306 + 912 WP_000705847.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T282885 WP_000227784.1 NZ_CP126345:3539303-3539845 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT282885 WP_001297137.1 NZ_CP126345:3539008-3539319 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References