Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1466072..1466697 Replicon chromosome
Accession NC_018650
Organism Escherichia coli O104:H4 str. 2009EL-2050

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag O3M_RS07335 Protein ID WP_000911329.1
Coordinates 1466299..1466697 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag O3M_RS07330 Protein ID WP_000450524.1
Coordinates 1466072..1466299 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3M_RS07300 1461874..1462344 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
O3M_RS07305 1462344..1462916 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
O3M_RS07310 1463062..1463940 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
O3M_RS07315 1463957..1464991 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
O3M_RS07325 1465204..1465917 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
O3M_RS07330 1466072..1466299 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
O3M_RS07335 1466299..1466697 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
O3M_RS07340 1466844..1467707 + 864 WP_001267498.1 neutral zinc metallopeptidase -
O3M_RS07345 1467722..1469737 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
O3M_RS07350 1469811..1470509 + 699 WP_000679823.1 esterase -
O3M_RS07355 1470619..1470819 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T28285 WP_000911329.1 NC_018650:1466299-1466697 [Escherichia coli O104:H4 str. 2009EL-2050]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T28285 NC_018650:1466299-1466697 [Escherichia coli O104:H4 str. 2009EL-2050]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT28285 WP_000450524.1 NC_018650:1466072-1466299 [Escherichia coli O104:H4 str. 2009EL-2050]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT28285 NC_018650:1466072-1466299 [Escherichia coli O104:H4 str. 2009EL-2050]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References