Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1021369..1022023 Replicon chromosome
Accession NC_018650
Organism Escherichia coli O104:H4 str. 2009EL-2050

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag O3M_RS05170 Protein ID WP_000244772.1
Coordinates 1021616..1022023 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag O3M_RS05165 Protein ID WP_000354046.1
Coordinates 1021369..1021635 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3M_RS05140 1016656..1017399 + 744 WP_000951964.1 SDR family oxidoreductase -
O3M_RS05145 1017456..1018889 - 1434 WP_001387034.1 6-phospho-beta-glucosidase BglA -
O3M_RS05150 1018934..1019245 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
O3M_RS05155 1019409..1020068 + 660 WP_000250274.1 hemolysin III family protein -
O3M_RS05160 1020146..1021126 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
O3M_RS05165 1021369..1021635 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
O3M_RS05170 1021616..1022023 + 408 WP_000244772.1 protein YgfX Toxin
O3M_RS05175 1022063..1022584 - 522 WP_001055874.1 flavodoxin FldB -
O3M_RS05180 1022696..1023592 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
O3M_RS05185 1023617..1024327 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
O3M_RS05190 1024333..1026066 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T28283 WP_000244772.1 NC_018650:1021616-1022023 [Escherichia coli O104:H4 str. 2009EL-2050]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T28283 NC_018650:1021616-1022023 [Escherichia coli O104:H4 str. 2009EL-2050]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT28283 WP_000354046.1 NC_018650:1021369-1021635 [Escherichia coli O104:H4 str. 2009EL-2050]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT28283 NC_018650:1021369-1021635 [Escherichia coli O104:H4 str. 2009EL-2050]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References