Detailed information of TA system TA028278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1625085..1625755
• Replicon: chromosome
• Accession: NC_018649
• Organism: Leptospirillum ferriphilum ML-04

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: J9ZDJ7
• Locus tag: LFML04_RS08265
• Protein ID: WP_014961408.1
• Coordinates: 1625327..1625755 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J9ZE31
• Locus tag: LFML04_RS08260
• Protein ID: WP_014961407.1
• Coordinates: 1625085..1625327 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFML04_RS08235	1620332..1621246	+	915	Protein_1538	site-specific integrase	-
LFML04_RS08240	1621323..1622543	-	1221	WP_014961401.1	ISNCY family transposase	-
LFML04_RS08245	1622686..1622946	+	261	WP_014961402.1	hypothetical protein	-
LFML04_RS08250	1623058..1623273	-	216	WP_014961403.1	hypothetical protein	-
LFML04_RS12820	1623797..1624360	-	564	WP_014961404.1	SocA family protein	-
LFML04_RS08260	1625085..1625327	+	243	WP_014961407.1	hypothetical protein	Antitoxin
LFML04_RS08265	1625327..1625755	+	429	WP_014961408.1	PIN domain-containing protein	Toxin
LFML04_RS13560	1626155..1626244	-	90	Protein_1545	IS1595 family transposase	-
LFML04_RS13775	1627060..1627662	+	603	WP_187288726.1	hypothetical protein	-
LFML04_RS13780	1627873..1628034	+	162	WP_014961412.1	hypothetical protein	-
LFML04_RS08290	1628879..1629880	-	1002	WP_014961415.1	hypothetical protein	-
LFML04_RS08295	1630205..1630525	+	321	WP_014961417.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1582031..1795193	213162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15814.97 Da
• Isoelectric Point: 6.7177

>T28278 WP_014961408.1 NC_018649:1625327-1625755 [Leptospirillum ferriphilum ML-04]
MRSLLDINVLIALLDSDHVSHHTAREWFSLHATDGWASCPLTQNGCMRIMSHPNYPNPIPISRIRERLSEAASSSLHQFW
PDDVSFLDAGVADTARIHGPHQITDLYLLALAIRHKGRFVTFDGSIVTSAVQGFEGHHLVVL

Nucleotide

• Length: 429 bp

>T28278 NC_018649:1625327-1625755 [Leptospirillum ferriphilum ML-04]
ATGCGTTCTCTCCTCGACATCAATGTTCTGATTGCTCTTCTGGATTCCGATCACGTTTCTCATCATACAGCCAGAGAGTG
GTTTTCTCTCCACGCAACCGATGGATGGGCCTCCTGTCCTTTAACACAGAACGGTTGCATGCGCATCATGTCCCATCCGA
ACTATCCAAACCCGATTCCCATCTCCCGGATCCGGGAACGTTTGTCCGAAGCAGCATCGAGTTCCCTGCATCAATTTTGG
CCGGATGACGTCAGTTTTCTGGACGCTGGCGTCGCAGATACCGCCCGTATCCATGGTCCACACCAGATCACCGACCTGTA
TCTCCTGGCCCTCGCCATCCGGCATAAGGGCCGTTTTGTCACCTTTGACGGTTCGATTGTGACCTCCGCCGTGCAGGGCT
TCGAAGGGCATCATCTGGTCGTTCTCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9025.20 Da
• Isoelectric Point: 5.1468

>AT28278 WP_014961407.1 NC_018649:1625085-1625327 [Leptospirillum ferriphilum ML-04]
MRTTLDIDEDVLETARELARRQRKSIGKVISDLGRQALMEMSGRDLSSETVSVFGFRPFPKRGGVVTNDRIDALREDDLS

Nucleotide

• Length: 243 bp

>AT28278 NC_018649:1625085-1625327 [Leptospirillum ferriphilum ML-04]
ATGAGAACGACGCTGGATATTGATGAGGATGTCCTGGAGACAGCCCGGGAATTGGCTCGTCGACAAAGGAAATCCATTGG
AAAAGTGATTTCCGATCTTGGGCGACAGGCATTGATGGAAATGTCGGGGAGGGACTTGTCGTCCGAGACGGTTTCTGTGT
TCGGGTTTCGTCCTTTTCCCAAAAGAGGAGGCGTGGTGACAAATGACCGGATAGATGCTTTGCGGGAAGACGATCTCTCC
TGA

Structures

Toxin

• AlphaFold DB: J9ZDJ7

Antitoxin

• AlphaFold DB: J9ZE31