28277

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapB(antitoxin)
Location 304160..304731 Replicon chromosome
Accession NC_018649
Organism Leptospirillum ferriphilum ML-04

Toxin (Protein)


Gene name vapC Uniprot ID J9Z9G6
Locus tag LFML04_RS01595 Protein ID WP_014960093.1
Coordinates 304160..304537 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A059XSE8
Locus tag LFML04_RS01600 Protein ID WP_038506638.1
Coordinates 304534..304731 (-) Length 66 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LFML04_RS01555 299659..300237 + 579 WP_014960087.1 hypothetical protein -
LFML04_RS01560 300265..301527 - 1263 WP_014960088.1 MFS transporter -
LFML04_RS01570 301906..302565 + 660 WP_101494852.1 oxidoreductase -
LFML04_RS01580 302874..303305 - 432 WP_014960090.1 putative toxin-antitoxin system toxin component, PIN family -
LFML04_RS01585 303312..303605 - 294 WP_014960091.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LFML04_RS12425 303770..304099 + 330 WP_014960092.1 hypothetical protein -
LFML04_RS01595 304160..304537 - 378 WP_014960093.1 type II toxin-antitoxin system VapC family toxin Toxin
LFML04_RS01600 304534..304731 - 198 WP_038506638.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LFML04_RS01605 304961..305896 - 936 WP_014960095.1 radical SAM protein -
LFML04_RS01610 305884..306870 - 987 WP_014960096.1 thiamine biosynthesis protein -
LFML04_RS01615 306867..307181 - 315 WP_014960097.1 antibiotic biosynthesis monooxygenase -
LFML04_RS01620 307669..308837 + 1169 Protein_297 ATP-binding protein -
LFML04_RS12430 309186..309671 - 486 Protein_298 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-112)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-46)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14256.43 Da        Isoelectric Point: 6.7422

>T28277 WP_014960093.1 NC_018649:c304537-304160 [Leptospirillum ferriphilum ML-04]
MILVDTSVWIDHFRKGNHRLKKILEQESASMHDFVLGELALGNFRDRQNVLSLLTSLPRCPCANPREILWLVEHHRLMGK
GVGYVDACLLASARLTGFRLWTKDLRLAAMARELECDDHESEDGS

Download         Length: 378 bp

>T28277 NC_018649:c304537-304160 [Leptospirillum ferriphilum ML-04]
ATGATTCTCGTCGATACATCGGTATGGATCGACCATTTCCGGAAGGGTAACCATCGTCTCAAGAAGATTTTGGAACAAGA
ATCCGCTTCCATGCATGATTTTGTTCTTGGCGAACTGGCCTTGGGCAATTTTCGAGATCGCCAGAACGTCCTCTCCCTCC
TCACATCCCTCCCACGATGTCCTTGCGCCAACCCTAGAGAAATCCTCTGGCTGGTGGAGCATCACCGGCTGATGGGAAAG
GGCGTCGGATATGTGGATGCTTGCCTGCTCGCTTCCGCAAGATTGACAGGATTCCGGCTGTGGACAAAAGACCTGCGACT
GGCCGCGATGGCCCGTGAACTCGAGTGCGACGATCATGAATCAGAGGATGGATCGTGA

Antitoxin


Download         Length: 66 a.a.        Molecular weight: 7350.46 Da        Isoelectric Point: 8.8949

>AT28277 WP_038506638.1 NC_018649:c304731-304534 [Leptospirillum ferriphilum ML-04]
MRTTVQIDESLLSLAQDITGITSRNAILKEALEALIQRESARRLARLGGSEPQMMDIPRRKNEKE

Download         Length: 198 bp

>AT28277 NC_018649:c304731-304534 [Leptospirillum ferriphilum ML-04]
ATGAGAACGACCGTGCAGATCGATGAATCCCTTTTATCCCTGGCTCAGGATATAACTGGCATCACCAGTCGGAACGCGAT
CCTGAAAGAAGCGCTGGAAGCGCTGATCCAGAGGGAAAGTGCCCGGCGTCTGGCGAGGCTGGGAGGCAGCGAACCCCAAA
TGATGGATATTCCAAGACGGAAGAATGAGAAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J9Z9G6

Antitoxin

Source ID Structure
AlphaFold DB A0A059XSE8

References