Detailed information of TA system TA028274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 3075076..3075601
• Replicon: chromosome
• Accession: NC_018645
• Organism: Desulfobacula toluolica Tol2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: K0NLA8
• Locus tag: TOL2_RS14035
• Protein ID: WP_014958075.1
• Coordinates: 3075317..3075601 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: K0NPI6
• Locus tag: TOL2_RS14030
• Protein ID: WP_014957534.1
• Coordinates: 3075076..3075327 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOL2_RS14015	3070733..3071794	+	1062	WP_014958073.1	hypothetical protein	-
TOL2_RS14020	3071805..3072386	+	582	WP_198408338.1	NADAR family protein	-
TOL2_RS14025	3072997..3074337	-	1341	WP_014957509.1	group II intron reverse transcriptase/maturase	-
TOL2_RS24915	3074740..3074904	+	165	WP_158406100.1	hypothetical protein	-
TOL2_RS14030	3075076..3075327	+	252	WP_014957534.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
TOL2_RS14035	3075317..3075601	+	285	WP_014958075.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TOL2_RS14040	3076350..3077435	-	1086	WP_014958076.1	transposase	-
TOL2_RS14045	3077432..3078307	-	876	WP_014957515.1	site-specific integrase	-
TOL2_RS23685	3078396..3079196	+	801	WP_051012398.1	GrpB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (11-77)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10980.94 Da
• Isoelectric Point: 10.5696

>T28274 WP_014958075.1 NC_018645:3075317-3075601 [Desulfobacula toluolica Tol2]
MSYKLKFLPTALKEWKKLDNSILAQFRKKLKERLEVPHVPSGKLSGFENHYKIKLRASGYRLVYEVIDQELFVLVVAVGK
REKGAVYEKARDRS

Nucleotide

• Length: 285 bp

>T28274 NC_018645:3075317-3075601 [Desulfobacula toluolica Tol2]
ATGAGCTATAAACTAAAGTTTCTCCCAACGGCTCTGAAGGAATGGAAGAAGCTGGATAATTCTATTTTGGCGCAATTCAG
GAAGAAACTGAAAGAGCGTTTGGAGGTACCTCATGTACCCTCCGGTAAGCTTTCCGGCTTTGAGAATCATTACAAAATCA
AACTTAGGGCCAGTGGGTATCGACTTGTGTATGAAGTGATCGATCAAGAATTGTTCGTCCTTGTTGTTGCTGTTGGAAAA
AGAGAAAAAGGTGCTGTGTATGAAAAAGCACGAGACAGATCATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9146.35 Da
• Isoelectric Point: 4.2333

>AT28274 WP_014957534.1 NC_018645:3075076-3075327 [Desulfobacula toluolica Tol2]
MEAVLANCSASISELKKNPSALIDASGGEPIAILNHNKPTAYLIPADTYQQILDRLEDYELGLIVKEREHEKAQAIEVDI
NEL

Nucleotide

• Length: 252 bp

>AT28274 NC_018645:3075076-3075327 [Desulfobacula toluolica Tol2]
ATGGAGGCCGTATTAGCTAACTGTTCAGCAAGTATTTCTGAGTTAAAGAAAAACCCCTCAGCATTGATTGACGCATCAGG
CGGAGAACCCATTGCTATCTTAAACCACAACAAACCAACCGCTTACTTGATCCCAGCCGACACCTATCAGCAAATCCTTG
ATAGGTTGGAGGATTATGAACTCGGACTGATCGTTAAAGAGCGTGAACATGAGAAAGCCCAGGCCATTGAGGTAGATATC
AATGAGCTATAA

Structures

Toxin

• AlphaFold DB: K0NLA8

Antitoxin

• AlphaFold DB: K0NPI6