Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 489236..489873 Replicon chromosome
Accession NZ_CP126226
Organism Bacillus velezensis strain B31

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QNM98_RS02495 Protein ID WP_003156187.1
Coordinates 489523..489873 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag QNM98_RS02490 Protein ID WP_003156188.1
Coordinates 489236..489517 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QNM98_RS02470 (QNM98_02470) 485601..486200 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
QNM98_RS02475 (QNM98_02475) 486293..486658 + 366 WP_007410229.1 holo-ACP synthase -
QNM98_RS02480 (QNM98_02480) 486823..487830 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
QNM98_RS02485 (QNM98_02485) 487947..489116 + 1170 WP_012116870.1 alanine racemase -
QNM98_RS02490 (QNM98_02490) 489236..489517 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QNM98_RS02495 (QNM98_02495) 489523..489873 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QNM98_RS02500 (QNM98_02500) 489991..490812 + 822 WP_015416874.1 STAS domain-containing protein -
QNM98_RS02505 (QNM98_02505) 490817..491182 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
QNM98_RS02510 (QNM98_02510) 491185..491586 + 402 WP_003156178.1 anti-sigma regulatory factor -
QNM98_RS02515 (QNM98_02515) 491598..492605 + 1008 WP_015416875.1 PP2C family protein-serine/threonine phosphatase -
QNM98_RS02520 (QNM98_02520) 492669..492998 + 330 WP_033575044.1 anti-sigma factor antagonist -
QNM98_RS02525 (QNM98_02525) 492995..493477 + 483 WP_007609591.1 anti-sigma B factor RsbW -
QNM98_RS02530 (QNM98_02530) 493443..494231 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
QNM98_RS02535 (QNM98_02535) 494231..494833 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T282732 WP_003156187.1 NZ_CP126226:489523-489873 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT282732 WP_003156188.1 NZ_CP126226:489236-489517 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References