Detailed information of TA system TA028273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 2413342..2414012
• Replicon: chromosome
• Accession: NC_018645
• Organism: Desulfobacula toluolica Tol2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: K0NK00
• Locus tag: TOL2_RS11100
• Protein ID: WP_014957543.1
• Coordinates: 2413593..2414012 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: K0NNA9
• Locus tag: TOL2_RS11095
• Protein ID: WP_014957542.1
• Coordinates: 2413342..2413596 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOL2_RS25075	2408464..2408643	+	180	WP_162138510.1	hypothetical protein	-
TOL2_RS11065	2408587..2408850	-	264	WP_014957539.1	type II toxin-antitoxin system RelE/ParE family toxin	-
TOL2_RS11070	2408854..2409105	-	252	WP_014957540.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
TOL2_RS11075	2409286..2410626	-	1341	WP_014957541.1	group II intron reverse transcriptase/maturase	-
TOL2_RS11080	2411029..2411220	+	192	WP_014957532.1	hypothetical protein	-
TOL2_RS11085	2411217..2412557	-	1341	WP_014957509.1	group II intron reverse transcriptase/maturase	-
TOL2_RS25160	2412960..2413100	+	141	WP_173391125.1	hypothetical protein	-
TOL2_RS11095	2413342..2413596	+	255	WP_014957542.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
TOL2_RS11100	2413593..2414012	+	420	WP_014957543.1	type II toxin-antitoxin system VapC family toxin	Toxin
TOL2_RS11110	2414074..2415239	+	1166	Protein_2161	IS3 family transposase	-
TOL2_RS11115	2415791..2417395	+	1605	Protein_2162	IS1634 family transposase	-
TOL2_RS11120	2417474..2417806	+	333	WP_014957547.1	IS66 family insertion sequence hypothetical protein	-
TOL2_RS11125	2417803..2418159	+	357	WP_083863578.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15737.39 Da
• Isoelectric Point: 8.0492

>T28273 WP_014957543.1 NC_018645:2413593-2414012 [Desulfobacula toluolica Tol2]
MNYLLDTCVISELIKKQPNPKVVQWISNIEEASLFISVLTIGELHKGIEKLPESKKKNRLHKWVTYDLKKRFENKIIDFD
LQTATIWGKIQANSELLGQTLPAIDALIASTGISHGLIVVTRNTKDMEISGVDLLNPWL

Nucleotide

• Length: 420 bp

>T28273 NC_018645:2413593-2414012 [Desulfobacula toluolica Tol2]
ATGAACTACTTATTAGACACATGTGTTATCTCAGAATTAATAAAAAAACAGCCAAACCCAAAAGTGGTTCAATGGATATC
AAATATTGAAGAAGCAAGCTTATTCATAAGTGTACTCACAATAGGTGAACTTCATAAAGGAATAGAGAAATTACCAGAGA
GCAAGAAAAAAAATAGACTTCACAAATGGGTTACTTATGACCTAAAAAAACGGTTTGAAAATAAAATTATTGACTTTGAT
CTCCAAACAGCAACTATTTGGGGTAAAATTCAAGCCAATTCAGAATTATTGGGCCAGACATTACCCGCTATTGATGCTCT
TATTGCTTCTACAGGAATATCGCATGGCCTTATTGTTGTAACAAGGAATACTAAGGACATGGAAATCAGTGGGGTAGACT
TGCTTAATCCCTGGTTATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9832.40 Da
• Isoelectric Point: 9.8980

>AT28273 WP_014957542.1 NC_018645:2413342-2413596 [Desulfobacula toluolica Tol2]
MIENYWQLQDAKNKFSNLVETAQQKGPQIVTKHGKEAVVILSIKEYKRLTKPKINLVQFFKNSPLAEEMIEFSRNKDIPR
DVDL

Nucleotide

• Length: 255 bp

>AT28273 NC_018645:2413342-2413596 [Desulfobacula toluolica Tol2]
ATGATTGAAAACTATTGGCAACTTCAAGATGCAAAAAACAAATTTAGCAATCTTGTTGAAACTGCGCAGCAGAAAGGTCC
TCAAATTGTAACCAAACATGGTAAAGAGGCTGTAGTTATTCTTTCAATTAAAGAATATAAAAGGTTAACCAAACCCAAAA
TAAATTTGGTTCAATTTTTTAAAAATTCTCCTCTTGCAGAAGAAATGATTGAATTTTCGAGAAACAAAGATATTCCGAGA
GATGTCGACTTATGA

Structures

Toxin

• AlphaFold DB: K0NK00

Antitoxin

• AlphaFold DB: K0NNA9