Detailed information of TA system TA028271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2392324..2392910
• Replicon: chromosome
• Accession: NC_018645
• Organism: Desulfobacula toluolica Tol2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: K0NGW4
• Locus tag: TOL2_RS10970
• Protein ID: WP_014957528.1
• Coordinates: 2392324..2392578 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: K0NG94
• Locus tag: TOL2_RS10975
• Protein ID: WP_014957529.1
• Coordinates: 2392575..2392910 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOL2_RS10950	2388221..2389096	-	876	WP_014957525.1	site-specific integrase	-
TOL2_RS10955	2389383..2389682	+	300	WP_041279433.1	hypothetical protein	-
TOL2_RS10960	2390032..2391117	-	1086	WP_014957526.1	transposase	-
TOL2_RS10965	2391114..2391989	-	876	WP_014957527.1	site-specific integrase	-
TOL2_RS10970	2392324..2392578	+	255	WP_014957528.1	type II toxin-antitoxin system HicA family toxin	Toxin
TOL2_RS10975	2392575..2392910	+	336	WP_014957529.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
TOL2_RS10980	2393152..2394492	-	1341	WP_014957509.1	group II intron reverse transcriptase/maturase	-
TOL2_RS24125	2394895..2395260	+	366	WP_014957530.1	hypothetical protein	-
TOL2_RS10990	2395599..2396939	-	1341	WP_014957509.1	group II intron reverse transcriptase/maturase	-
TOL2_RS10995	2397342..2397533	+	192	WP_014957532.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-73)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9626.23 Da
• Isoelectric Point: 10.3631

>T28271 WP_014957528.1 NC_018645:2392324-2392578 [Desulfobacula toluolica Tol2]
MKTKHQKTLATIFTDPVNGNMEWNKIEALLIALGCRVIEGAGSSVTFEKKGIRAYFHRPHPEKEALRYRIKDAREFIKKI
GETP

Nucleotide

• Length: 255 bp

>T28271 NC_018645:2392324-2392578 [Desulfobacula toluolica Tol2]
ATGAAAACAAAACACCAAAAAACTTTGGCAACAATTTTCACTGACCCTGTAAATGGGAATATGGAATGGAACAAGATTGA
GGCGCTTCTTATCGCACTTGGTTGCCGAGTTATTGAGGGAGCAGGATCAAGCGTTACCTTTGAGAAAAAAGGCATTCGAG
CATATTTTCACAGACCACATCCTGAGAAGGAAGCTTTGAGGTATCGCATTAAGGATGCTCGTGAATTCATAAAGAAAATA
GGAGAGACACCATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12080.49 Da
• Isoelectric Point: 5.1253

>AT28271 WP_014957529.1 NC_018645:2392575-2392910 [Desulfobacula toluolica Tol2]
MKGMNHKGYFAKVEFDSEDHIFVGHIIGIRDIVGFHGESVTELETAFQEAVDNYLAACNELGQEPNKPYSGNLMLRIPSE
IHAAVASAAEASGKSINQWAAGVLEKESHAH

Nucleotide

• Length: 336 bp

>AT28271 NC_018645:2392575-2392910 [Desulfobacula toluolica Tol2]
ATGAAAGGGATGAATCATAAAGGCTATTTCGCCAAAGTTGAATTTGACTCTGAGGATCATATCTTTGTCGGCCATATTAT
TGGCATCCGTGATATTGTTGGTTTTCATGGTGAATCTGTCACCGAGTTGGAAACAGCCTTTCAAGAGGCAGTTGACAACT
ACCTTGCTGCCTGTAACGAACTTGGCCAAGAGCCTAACAAGCCATATTCCGGCAATCTTATGCTAAGAATTCCTTCCGAG
ATACATGCGGCAGTGGCTTCTGCAGCAGAAGCCAGCGGTAAAAGCATTAACCAGTGGGCGGCTGGAGTTCTGGAGAAAGA
AAGTCACGCCCATTAA

Structures

Toxin

• AlphaFold DB: K0NGW4

Antitoxin

• AlphaFold DB: K0NG94