Detailed information of TA system TA028270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2359453..2360045
• Replicon: chromosome
• Accession: NC_018645
• Organism: Desulfobacula toluolica Tol2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: TOL2_RS10810
• Protein ID: WP_041279425.1
• Coordinates: 2359453..2359641 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: K0N8F5
• Locus tag: TOL2_RS10815
• Protein ID: WP_014957507.1
• Coordinates: 2359644..2360045 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOL2_RS10790	2354830..2355840	+	1011	WP_014957503.1	tryptophan--tRNA ligase	-
TOL2_RS10795	2355829..2356521	-	693	WP_014957504.1	rRNA pseudouridine synthase	-
TOL2_RS10800	2356524..2357693	-	1170	WP_014957505.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
TOL2_RS10805	2358229..2359236	-	1008	WP_198408370.1	integron integrase	-
TOL2_RS10810	2359453..2359641	+	189	WP_041279425.1	type II toxin-antitoxin system HicA family toxin	Toxin
TOL2_RS10815	2359644..2360045	+	402	WP_014957507.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
TOL2_RS23605	2360196..2360756	+	561	WP_083863568.1	hypothetical protein	-
TOL2_RS10825	2360848..2362188	-	1341	WP_014957509.1	group II intron reverse transcriptase/maturase	-
TOL2_RS10830	2362591..2362782	+	192	WP_014957532.1	hypothetical protein	-
TOL2_RS10835	2362779..2364119	-	1341	WP_014957509.1	group II intron reverse transcriptase/maturase	-
TOL2_RS24870	2364522..2364686	+	165	WP_158406100.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7358.52 Da
• Isoelectric Point: 11.4539

>T28270 WP_041279425.1 NC_018645:2359453-2359641 [Desulfobacula toluolica Tol2]
MNNRDIIRRLKSDGWILHHAKGDHHQFKHPAKKGKVTIPHPKKDLAIGTLRNIYRQAGWDWR

Nucleotide

• Length: 189 bp

>T28270 NC_018645:2359453-2359641 [Desulfobacula toluolica Tol2]
ATGAACAACCGAGACATAATTCGAAGGTTAAAAAGCGATGGATGGATTCTCCATCATGCTAAAGGAGATCATCACCAATT
TAAGCATCCAGCTAAAAAAGGGAAAGTAACCATTCCTCATCCTAAAAAGGATTTAGCTATTGGAACTTTACGAAATATTT
ATCGGCAAGCCGGGTGGGATTGGAGGTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14904.87 Da
• Isoelectric Point: 4.7881

>AT28270 WP_014957507.1 NC_018645:2359644-2360045 [Desulfobacula toluolica Tol2]
MKYSIVIHKDPDSDYSITIPDLPGCYSAGETIEEAINMAQEAAECHIEGMLIDSEPIPTTTDIEKHKDDPNYKNGTWALI
EVDISKLSLKSKRINITMPERLLRTVDQYAKKYGSSRSGLLSQAITEYMAAHQ

Nucleotide

• Length: 402 bp

>AT28270 NC_018645:2359644-2360045 [Desulfobacula toluolica Tol2]
ATGAAATATTCCATCGTTATTCATAAAGACCCAGATTCAGATTATAGCATAACTATTCCTGATCTTCCGGGTTGTTACTC
TGCAGGAGAGACTATCGAAGAAGCAATTAATATGGCACAGGAAGCAGCAGAGTGTCATATTGAAGGAATGCTTATTGATT
CAGAACCTATTCCAACAACTACCGATATTGAAAAGCACAAAGATGATCCCAATTATAAAAATGGAACTTGGGCACTAATT
GAGGTAGATATAAGTAAGCTCTCATTAAAATCGAAACGCATAAATATTACAATGCCTGAAAGATTACTAAGAACTGTTGA
TCAATATGCAAAAAAATATGGGTCCAGCAGATCAGGCCTACTGTCTCAAGCTATTACTGAATATATGGCTGCACACCAAT
AA

Structures

Antitoxin

• AlphaFold DB: K0N8F5