Detailed information of TA system TA028269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1508340..1509991
• Replicon: chromosome
• Accession: NC_018645
• Organism: Desulfobacula toluolica Tol2

Toxin (Protein)

• Gene name: HipA3
• Uniprot ID: K0NL50
• Locus tag: TOL2_RS06900
• Protein ID: WP_014956789.1
• Coordinates: 1508672..1509991 (+)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: HipB3
• Uniprot ID: -
• Locus tag: TOL2_RS06895
• Protein ID: WP_014956788.1
• Coordinates: 1508340..1508678 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOL2_RS06860	1503697..1504257	-	561	WP_014956784.1	recombinase family protein	-
TOL2_RS06865	1504554..1504754	-	201	WP_041279332.1	hypothetical protein	-
TOL2_RS06870	1504785..1505264	-	480	WP_014956785.1	hypothetical protein	-
TOL2_RS06875	1505301..1505582	-	282	WP_041279334.1	DNA-binding protein	-
TOL2_RS06880	1505841..1506614	-	774	WP_041279336.1	hypothetical protein	-
TOL2_RS24600	1506795..1506980	+	186	WP_148278071.1	hypothetical protein	-
TOL2_RS06885	1507064..1507921	+	858	WP_014956787.1	sigma 54-interacting transcriptional regulator	-
TOL2_RS06895	1508340..1508678	+	339	WP_014956788.1	helix-turn-helix domain-containing protein	Antitoxin
TOL2_RS06900	1508672..1509991	+	1320	WP_014956789.1	type II toxin-antitoxin system HipA family toxin	Toxin
TOL2_RS06905	1510091..1510315	+	225	WP_014956790.1	hypothetical protein	-
TOL2_RS06910	1510312..1510734	+	423	WP_014956791.1	virulence RhuM family protein	-
TOL2_RS06915	1510957..1512288	+	1332	WP_014956792.1	cell envelope integrity protein CreD	-
TOL2_RS06920	1512459..1513808	+	1350	WP_041279865.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-117)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-66)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49735.98 Da
• Isoelectric Point: 9.7630

>T28269 WP_014956789.1 NC_018645:1508672-1509991 [Desulfobacula toluolica Tol2]
MVIEIVKVKYREQDVGAISFNTKTGVGAFEFESRFIKSGIELSPVKMPLSKKIYSFPENNNETFKGLPGMIADSLPDDFG
NAVMNAWIAGQGKSTTDITPLQRLQYTGKRGMGALTYSPAIQRKNLNRSQHVDIQSLIFIAQEILDKREQFQVTLGPQGQ
EDKEAMTALLSVGTSAGGARPKAVLAFNSDFTQVRSGQTNAPEGFTHCVMKFDGVSEIHKNKETFGDPLGYGTMEYVYHL
MAKACGIDMMPCRLLNEGNRRHFITQRFDRKGNRKIHVQTLNGIAHVDYKKVGSFSYEELFSLARELKLTPDDAIQIFKR
MVFNIVARNHDDHSKNFGFMLDNQNKWQLAPAYDLAYSYKPGSPWVSSHWMKLNGKRDNFIRKDFYSLKKISPIFTKRKI
DHIIEEIIEHVSQWDDLAVAQGVPQSLIKSISSNLRLKF

Nucleotide

• Length: 1320 bp

>T28269 NC_018645:1508672-1509991 [Desulfobacula toluolica Tol2]
ATGGTAATCGAAATCGTAAAAGTAAAATATAGAGAACAGGATGTCGGTGCCATAAGTTTTAATACCAAAACAGGTGTTGG
CGCATTTGAATTTGAATCTCGCTTTATTAAGTCGGGTATTGAGCTTTCACCTGTAAAAATGCCTTTGTCTAAAAAGATTT
ATTCGTTTCCGGAGAATAATAACGAAACTTTCAAAGGATTACCCGGGATGATTGCAGATTCTTTGCCTGATGATTTTGGT
AATGCAGTGATGAATGCCTGGATAGCCGGTCAGGGGAAATCTACAACAGACATCACCCCATTGCAGCGTCTTCAATATAC
AGGAAAGCGTGGCATGGGAGCATTAACTTATTCTCCGGCAATCCAGCGGAAAAATCTGAACAGGTCACAACATGTTGACA
TTCAGTCTTTAATTTTCATTGCCCAGGAAATCTTGGACAAACGGGAACAATTCCAGGTAACGCTTGGACCACAAGGCCAA
GAAGATAAAGAAGCTATGACGGCACTGTTATCTGTCGGTACGAGTGCCGGTGGTGCCAGACCTAAAGCAGTGCTTGCATT
TAACAGTGATTTTACTCAGGTGAGGTCGGGGCAAACCAATGCGCCTGAAGGTTTTACTCATTGCGTGATGAAGTTTGACG
GTGTCAGCGAAATTCATAAAAATAAAGAAACATTTGGTGATCCATTGGGATACGGCACAATGGAGTATGTTTATCATCTC
ATGGCCAAAGCTTGTGGAATAGACATGATGCCCTGCCGGTTGCTCAACGAAGGGAATCGACGTCATTTCATTACTCAGCG
GTTTGACAGGAAAGGCAACCGGAAAATACATGTACAAACATTAAACGGTATTGCACATGTTGATTACAAAAAGGTCGGCT
CGTTTTCATATGAAGAATTGTTTTCACTTGCAAGAGAACTCAAGTTAACTCCGGATGATGCAATACAAATATTCAAACGA
ATGGTTTTTAATATTGTTGCACGCAATCATGACGACCATTCTAAAAATTTTGGATTCATGCTTGACAACCAAAACAAATG
GCAATTGGCACCAGCCTATGATTTGGCTTACAGCTACAAGCCTGGAAGCCCGTGGGTAAGCAGTCATTGGATGAAGCTCA
ATGGAAAGAGAGACAATTTTATCCGCAAAGATTTTTATAGCCTGAAAAAAATAAGCCCTATATTTACCAAACGTAAAATT
GATCATATCATTGAAGAAATCATAGAGCATGTTTCACAATGGGATGACCTGGCCGTTGCACAGGGTGTTCCACAATCATT
AATCAAATCAATAAGCAGCAACTTGCGGTTAAAATTTTAG

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12581.83 Da
• Isoelectric Point: 11.5022

>AT28269 WP_014956788.1 NC_018645:1508340-1508678 [Desulfobacula toluolica Tol2]
MMFEKMSPVAIAGQLGERLKQARLNADLTQAELASRTGLNRRTILNAEKGNVQLKNLIAILISLGMVEQINVFLPVQEIS
PLQLAKLKGKKRQRASRSQKRKSQISEDKSLW

Nucleotide

• Length: 339 bp

>AT28269 NC_018645:1508340-1508678 [Desulfobacula toluolica Tol2]
ATGATGTTTGAAAAAATGTCGCCAGTCGCAATTGCCGGGCAATTAGGGGAGAGACTAAAACAAGCCAGGCTGAATGCCGA
TTTAACACAGGCTGAACTGGCATCAAGAACCGGTTTAAACAGGAGGACTATTTTGAATGCTGAGAAAGGTAACGTCCAAT
TAAAAAACCTGATCGCAATTCTGATAAGTCTTGGCATGGTTGAGCAAATCAATGTGTTTTTACCGGTACAGGAAATCTCT
CCTTTACAGTTGGCTAAACTAAAAGGGAAAAAACGGCAGCGAGCTTCAAGATCTCAAAAAAGAAAATCCCAAATAAGTGA
AGATAAATCTTTATGGTAA

Structures

Toxin

• AlphaFold DB: K0NL50