Detailed information of TA system TA028267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 501285..501887
• Replicon: chromosome
• Accession: NC_018645
• Organism: Desulfobacula toluolica Tol2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: K0NCZ3
• Locus tag: TOL2_RS02290
• Protein ID: WP_014955942.1
• Coordinates: 501285..501581 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: TOL2_RS02295
• Protein ID: WP_014955943.1
• Coordinates: 501585..501887 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOL2_RS02275	497233..498882	+	1650	WP_014955939.1	DUF262 domain-containing protein	-
TOL2_RS02280	498885..500051	+	1167	WP_014955940.1	DUF3696 domain-containing protein	-
TOL2_RS02285	500051..501019	+	969	WP_014955941.1	hypothetical protein	-
TOL2_RS02290	501285..501581	+	297	WP_014955942.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TOL2_RS02295	501585..501887	+	303	WP_014955943.1	putative addiction module antidote protein	Antitoxin
TOL2_RS02300	502027..504615	+	2589	WP_014955944.1	hypothetical protein	-
TOL2_RS02305	504639..505646	+	1008	WP_014955945.1	dual specificity protein phosphatase family protein	-
TOL2_RS02310	505661..506023	-	363	WP_014955946.1	response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (7-96)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11341.01 Da
• Isoelectric Point: 9.7545

>T28267 WP_014955942.1 NC_018645:501285-501581 [Desulfobacula toluolica Tol2]
MYTVKTLPEFDKWLNSLKDRITRLRLSRRLDKAQRGNLGDVKPVGEGVFEMREYFGPGWRMYYAKRGETLIVMLGGGDKD
SQESDIAKAKQREAILED

Nucleotide

• Length: 297 bp

>T28267 NC_018645:501285-501581 [Desulfobacula toluolica Tol2]
ATGTATACGGTTAAAACATTACCCGAATTTGACAAATGGCTTAACAGCTTGAAAGACCGCATTACACGGCTTCGCCTAAG
CCGCCGCCTTGATAAAGCACAGCGTGGCAACCTCGGTGATGTAAAACCAGTGGGTGAAGGCGTCTTTGAAATGAGGGAAT
ATTTCGGTCCAGGCTGGAGAATGTACTACGCCAAGCGGGGTGAAACTCTGATCGTCATGCTTGGTGGGGGAGACAAGGAC
AGCCAGGAGAGCGACATTGCCAAAGCTAAACAGCGGGAAGCCATTTTGGAGGATTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10821.51 Da
• Isoelectric Point: 8.0310

>AT28267 WP_014955943.1 NC_018645:501585-501887 [Desulfobacula toluolica Tol2]
MKKRITDLPDFDMAQQLKNEEDIAAYITMVIEDGDASELAHALGVAAKARGMSEVAKSSGITREALYKALKPNAKPRFDT
INKVCAALGVRLIAQPANPH

Nucleotide

• Length: 303 bp

>AT28267 NC_018645:501585-501887 [Desulfobacula toluolica Tol2]
ATGAAAAAACGAATTACAGATCTGCCGGATTTTGATATGGCACAACAGCTTAAAAATGAGGAAGACATTGCTGCTTACAT
CACTATGGTGATTGAAGACGGTGATGCCTCCGAGCTGGCCCACGCTCTGGGGGTCGCTGCCAAGGCACGTGGTATGAGCG
AAGTTGCCAAATCTTCAGGAATAACCCGTGAAGCCCTGTATAAAGCGCTTAAACCAAACGCTAAACCTCGATTTGACACG
ATTAATAAGGTCTGTGCAGCCCTTGGCGTTCGTCTGATAGCTCAGCCTGCCAACCCTCATTGA

Structures

Toxin

• AlphaFold DB: K0NCZ3