Detailed information of TA system TA028263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1613303..1613965
• Replicon: chromosome
• Accession: NC_018630
• Organism: Streptococcus pneumoniae gamPNI0373

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HMPREF1038_RS08595
• Protein ID: WP_000192224.1
• Coordinates: 1613792..1613965 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: G0IC64
• Locus tag: HMPREF1038_RS08590
• Protein ID: WP_000961810.1
• Coordinates: 1613303..1613755 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HMPREF1038_RS08565 (HMPREF1038_01745)	1609193..1609936	-	744	WP_001188213.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
HMPREF1038_RS08570 (HMPREF1038_01746)	1609938..1610891	-	954	WP_000970781.1	50S ribosomal protein L11 methyltransferase	-
HMPREF1038_RS08575 (HMPREF1038_01747)	1611026..1611454	-	429	WP_000418168.1	NUDIX hydrolase	-
HMPREF1038_RS08580 (HMPREF1038_01748)	1611435..1612523	-	1089	WP_000719722.1	M50 family metallopeptidase	-
HMPREF1038_RS08585 (HMPREF1038_01749)	1612542..1613012	-	471	WP_000257097.1	DUF3013 family protein	-
HMPREF1038_RS08590 (HMPREF1038_01750)	1613303..1613755	-	453	WP_000961810.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HMPREF1038_RS08595 (HMPREF1038_01751)	1613792..1613965	-	174	WP_000192224.1	type II toxin-antitoxin system HicA family toxin	Toxin
HMPREF1038_RS08600 (HMPREF1038_01752)	1614108..1614287	-	180	WP_001809903.1	hypothetical protein	-
HMPREF1038_RS08605 (HMPREF1038_01753)	1614452..1614691	-	240	WP_000818079.1	hypothetical protein	-
HMPREF1038_RS08610 (HMPREF1038_01754)	1614961..1616232	+	1272	WP_001113215.1	replication-associated recombination protein A	-
HMPREF1038_RS08620 (HMPREF1038_01757)	1616777..1618096	-	1320	WP_001844947.1	glycoside hydrolase family 32 protein	-
HMPREF1038_RS08625 (HMPREF1038_01758)	1618106..1618951	-	846	WP_000819520.1	carbohydrate ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (17-114)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6386.51 Da
• Isoelectric Point: 10.8214

>T28263 WP_000192224.1 NC_018630:c1613965-1613792 [Streptococcus pneumoniae gamPNI0373]
MTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGELNKYTERGIGKQAGL

Nucleotide

• Length: 174 bp

>T28263 NC_018630:c1613965-1613792 [Streptococcus pneumoniae gamPNI0373]
ATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCATATTAA
AATGGAGAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAGGAAAGC
AAGCTGGGTTGTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16659.46 Da
• Isoelectric Point: 3.7220

>AT28263 WP_000961810.1 NC_018630:c1613755-1613303 [Streptococcus pneumoniae gamPNI0373]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Nucleotide

• Length: 453 bp

>AT28263 NC_018630:c1613755-1613303 [Streptococcus pneumoniae gamPNI0373]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Structures

Antitoxin

• PDB: 5YRZ
• AlphaFold DB: G0IC64