Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 512276..512912 Replicon chromosome
Accession NZ_CP126094
Organism Bacillus subtilis strain G2S2

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QNH31_RS02620 Protein ID WP_003156187.1
Coordinates 512562..512912 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag QNH31_RS02615 Protein ID WP_003225183.1
Coordinates 512276..512557 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QNH31_RS02595 (QNH31_02595) 508636..509235 - 600 WP_041850724.1 rhomboid family intramembrane serine protease -
QNH31_RS02600 (QNH31_02600) 509330..509695 + 366 WP_015252768.1 holo-ACP synthase -
QNH31_RS02605 (QNH31_02605) 509861..510877 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
QNH31_RS02610 (QNH31_02610) 510991..512160 + 1170 WP_015252766.1 alanine racemase -
QNH31_RS02615 (QNH31_02615) 512276..512557 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QNH31_RS02620 (QNH31_02620) 512562..512912 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QNH31_RS02625 (QNH31_02625) 513027..513851 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
QNH31_RS02630 (QNH31_02630) 513856..514221 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
QNH31_RS02635 (QNH31_02635) 514225..514626 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
QNH31_RS02640 (QNH31_02640) 514638..515645 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
QNH31_RS02645 (QNH31_02645) 515707..516036 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
QNH31_RS02650 (QNH31_02650) 516033..516515 + 483 WP_003234299.1 anti-sigma B factor RsbW -
QNH31_RS02655 (QNH31_02655) 516481..517269 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
QNH31_RS02660 (QNH31_02660) 517269..517868 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T282603 WP_003156187.1 NZ_CP126094:512562-512912 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT282603 WP_003225183.1 NZ_CP126094:512276-512557 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References