Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 207966..208602 Replicon chromosome
Accession NZ_CP126088
Organism Priestia flexa strain CCW CN82

Toxin (Protein)


Gene name mazF Uniprot ID A0A0V8JQ27
Locus tag QNH32_RS01140 Protein ID WP_025910606.1
Coordinates 208252..208602 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1N6ZQI9
Locus tag QNH32_RS01135 Protein ID WP_025910605.1
Coordinates 207966..208247 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QNH32_RS01110 (QNH32_01110) 203240..204208 + 969 WP_239429273.1 UV DNA damage repair endonuclease UvsE -
QNH32_RS01115 (QNH32_01115) 204244..204837 - 594 WP_283880482.1 rhomboid family intramembrane serine protease -
QNH32_RS01120 (QNH32_01120) 204929..205297 + 369 WP_025910601.1 holo-ACP synthase -
QNH32_RS01125 (QNH32_01125) 205411..206427 + 1017 WP_025910603.1 outer membrane lipoprotein carrier protein LolA -
QNH32_RS01130 (QNH32_01130) 206616..207788 + 1173 WP_061785870.1 alanine racemase -
QNH32_RS01135 (QNH32_01135) 207966..208247 + 282 WP_025910605.1 hypothetical protein Antitoxin
QNH32_RS01140 (QNH32_01140) 208252..208602 + 351 WP_025910606.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QNH32_RS01145 (QNH32_01145) 208776..209612 + 837 WP_061785869.1 RsbT co-antagonist protein RsbRA -
QNH32_RS01150 (QNH32_01150) 209609..209971 + 363 WP_035321022.1 STAS domain-containing protein -
QNH32_RS01155 (QNH32_01155) 209975..210376 + 402 WP_025910609.1 anti-sigma regulatory factor -
QNH32_RS01160 (QNH32_01160) 210388..211401 + 1014 WP_061785868.1 PP2C family protein-serine/threonine phosphatase -
QNH32_RS01165 (QNH32_01165) 211464..211796 + 333 WP_061785867.1 anti-sigma factor antagonist -
QNH32_RS01170 (QNH32_01170) 211793..212278 + 486 WP_163070556.1 anti-sigma B factor RsbW -
QNH32_RS01175 (QNH32_01175) 212244..213038 + 795 WP_163070555.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.94 Da        Isoelectric Point: 5.1442

>T282596 WP_025910606.1 NZ_CP126088:208252-208602 [Priestia flexa]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDDALQISVGLIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10654.20 Da        Isoelectric Point: 9.5610

>AT282596 WP_025910605.1 NZ_CP126088:207966-208247 [Priestia flexa]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSEFIYQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAES
EANHTVDRLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V8JQ27

Antitoxin

Source ID Structure
AlphaFold DB A0A1N6ZQI9

References